紫薇WOX家族基因鉴定及其对愈伤诱导的影响  

Identification of WOX Family Genes in Lagerstroemia indica and Their Effects on Callus Induction

作　　者：康佳音 池秀凤 申萍 王鑫 万志庭 蔡明 程堂仁 王佳 张启翔 潘会堂 KANG Jia-Yin;CHI Xiu-Feng;SHEN Ping;WANG Xin;WAN Zhi-Ting;CAI Ming;CHENG Tang-Ren;WANG Jia;ZHANG Qi-Xiang;PAN Hui-Tang(School of Landscape Architecture/Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular Breeding/National Engineering Research Center for Floriculture/Beijing Laboratory of Urban and Rural Ecological Environment,Beijing Forestry University,Beijing 100083,China)

机构地区：[1]北京林业大学园林学院/花卉种质创新与分子育种北京市重点实验室/国家花卉工程技术研究中心/城乡生态环境北京实验室,北京100083

出　　处：《农业生物技术学报》2024年第10期2255-2264,共10页Journal of Agricultural Biotechnology

基　　金：国家重点研发计划(2019YFD1001004);国家林业和草原局林业科学技术推广项目([2019]01)。

摘　　要：作为植物特有的转录因子,WUSCHEL同源异型盒(WUSCHEL-related homeobox,WOX)基因家族对植物的生长发育、组织器官发生和形成具有重要的调节作用。本研究利用生物信息学方法,在紫薇(Lagerstroemia indica)全基因组中鉴定出15个WOX基因家族成员,命名为LfiWOX1~LfiWOX15,分布在14条染色体上,家族成员间分子量和等电点有较大差异,亚细胞预测15个基因均定位于细胞核上。系统进化分析表明,15个基因共聚为3类,紫薇WOX家族成员中存在5个与拟南芥(Arabidopsis thaliana)、水稻(Oryza sativa)紧密相关的直系同源WOX成员,多个含有特定保守基序的WOX蛋白在进化树的同一分支上,所有家族成员都含有motif 1、motif 2和motif 5。WOX家族基因包含9种与植物生长发育、激素调控和逆境胁迫相关的顺式作用元件,不同成员含有不同的元件。qRT-PCR分析表明,与叶片相比,LfiWOX12、LfiWOX13、LfiWOX15在愈伤组织中表达量升高,是叶片中的1.5~1.9倍,其他基因在愈伤组织中表达量降低,是叶片中的0.1%~90%,说明LfiWOX12、LfiWOX13、LfiWOX15与愈伤形成相关。本研究为进一步研究紫薇WOX基因对愈伤诱导的调控提供了依据,为紫薇高效再生及遗传转化体系的建立提供参考。As plant specific transcription factors,WOX gene family plays an important role in regulating plant growth and development,tissue and organ genesis and formation.This study used bioinformatics methods to identify 15 members of WOX gene family in the whole genome of Lagerstroemia indica,named LfiWOX1~LfiWOX15,which were distributed on 14 chromosomes.There were large differences in molecular weight and isoelectric point between proteins.All subcellular predictions were located in the nucleus.Systemic evolution analysis showed that genes were classified into 3 categories,and 5 orthologous WOX members closely related to Arabidopsis thaliana and rice(Oryza sativa)WOX genes.Additionally,some WOX genes with specific motifs in L.indica were classified into the same branch on the evolutionary tree,and all family members contain motif 1,motif 2,and motif 5.The WOX family genes contain 9 cis-regulatory elements related to plant growth and development,hormone regulation,and stress,with different members containing different elements.qRT-PCR analysis showed that the expression levels of LfiWOX12,LfiWOX13 and LfiWOX15 in callus tissue increased by 1.5~1.9 times compared to leaves,while the expression levels of other genes in callus tissue decreased by 0.1~90% compared to leaves.It was speculated that LfiWOX12,LfiWOX13 and LfiWOX15 were related to callus formation.The results provide basic data for further studying the regulation of WOX gene on callus induction and theoretical reference for the establishment of efficient regeneration and genetic transformation system in L.indica.

关 键 词：紫薇 WUSCHEL同源异型盒基因家族(WOX) 愈伤诱导 基因表达 生物信息学分析 

分 类 号：S685.99[农业科学—观赏园艺]