利用组蛋白去乙酰化酶抑制剂提高猪体细胞核移植胚胎发育率的研究进展  

Research Progress on Improvement in Development of Porcine Somatic Cell Nuclear Transfer Embryos by Histone Deacetylase Inhibitors

作　　者：晏超 陈指龙 彭翠婷 谢浩 张才用 赵玉兰 齐霖 刘永刚 唐中林 YAN Chao;CHEN Zhi-Long;PENG Cui-Ting;XIE Hao;ZHANG Cai-Yong;ZHAO Yu-Lan;QI Lin;LIU Yong-Gang;TANG Zhong-Lin(College of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518000,China;Kunpeng Institute of Modern Agriculture at Foshan,Foshan 528226,China;Shenzhen Branch Center of Guangdong Laboratory of Lingnan Modern Agricultural Science and Technology,Shenzhen 518000,China;Key Laboratory of Livestock and Poultry Biohistology,Ministry of Agriculture and Rural Development,Shenzhen 518000,China)

机构地区：[1]云南农业大学动物科学技术学院,昆明650201 [2]中国农业科学院深圳农业基因组研究所,深圳518000 [3]佛山鲲鹏现代农业研究院,佛山528226 [4]岭南现代农业科学与技术广东省实验室深圳分中心,深圳518000 [5]农业农村部畜禽生物组学重点实验室,深圳518000

出　　处：《农业生物技术学报》2024年第10期2402-2412,共11页Journal of Agricultural Biotechnology

基　　金：广东省基础与应用基础研究基金委员会区域联合基金—青年基金项目(2021A1515110046);国家自然科学基金青年项目(32302747);深圳市可持续发展科技专项项目(KCXFZ20201221173213037)。

摘　　要：体细胞核移植(somatic cell nuclear transfer,SCNT)技术在保护濒危物种、扩繁良种和治疗疾病等方面应用广泛。组蛋白乙酰化修饰是调控猪(Sus scrofa)早期胚胎发育重要的表观遗传标记,其正确的擦除与重建是胚胎正常发育的基础。随着微量细胞测序技术的发展,研究人员发现体细胞克隆胚胎重编程过程中存在多种表观遗传修饰异常,其中组蛋白乙酰化修饰异常是导致猪体细胞克隆胚胎发育阻滞的重要因素。近年来,一些组蛋白去乙酰化酶抑制剂逐渐被发掘,可用于改善胚胎发育过程中组蛋白乙酰化的异常修饰水平。本文综述了近年来利用组蛋白去乙酰化酶抑制剂提高猪体细胞核移植胚胎发育及其作用机制的相关研究,为提高猪体细胞克隆效率提供参考。Somatic cell nuclear transfer(SCNT)has been widely used in protecting endangered species,propagating better varieties and treating diseases.Histone acetylase is an important epigenetic mark regulating the development of early embryo in pig(Sus scrofa),and its correct obliteration and reconstruction are the basis of the embryonic development.With the development of low input next generation sequencing,many studies found that a variety of abnormal epigenetic modification have been found during the reprogramming of somatic cell nuclear transfer embryos.Histone acetylase modification is an important factor that leads to the development block of porcine somatic cell nuclear transfer embryos.In recent years,some small molecule compounds of histone deacetylase inhibitors have been gradually discovered for the improvement of abnormal modification level of histone acetylase during embryonic development.This paper reviews the studies of the effects and mechanisms of histone deacetylase inhibitors on the development of porcine somatic cell nuclear transfer embryos,which provides reference for improving the cloning efficiency in pig.

关 键 词：猪 体细胞核移植(SCNT) 组蛋白去乙酰化酶抑制剂 胚胎 发育 

分 类 号：S814.6[农业科学—畜牧学]