对叶百部种子质量检验方法研究  

Study on Seed Quality Inspection Method of Stemona tuberosa Lour.

作　　者：石瑶[1] 汤红丽 孔凡旭 胡明举 徐杰[3] 袁理春[1] SHI Yao;TANG Hongli;KONG Fanxu;HU Mingju;XU Jie;YUAN Lichun(Institute of Medical Plant,Yunnan Academy of Agricultural Sciences,Kunming 650200,China;College of Tropical Crops,Yunnan Agricultural University,Pu'er Yunnan 665099,China;Wenshan Academy of Agricultural Sciences,Wenshan Yunnan 663000,China)

机构地区：[1]云南省农业科学院药用植物研究所,昆明650200 [2]云南农业大学热带作物学院,云南普洱665099 [3]文山州农业科学院,云南文山663000

出　　处：《种子》2024年第9期116-121,共6页Seed

基　　金：科技部、财政部国家科技资源共享服务平台项目“国家作物种质资源库-食药两用资源整合与共享分库”(NCGRC-2023-55);云南省地方标准制定项目“对叶百部栽培技术规程”。

摘　　要：以云南省文山、楚雄、普洱等地对叶百部种子为材料,参照GB/T 3543-1995《农作物种子检验规程》,研究对叶百部种子质量检验方法。结果表明,种子净度分析最少试验量为75 g,送检样品量不少于750 g;真实性鉴定采用种子外观形态比较;质量测定采用百粒法;水分含量测定采用高恒温(130±2)℃烘干5 h;种子生活力测定采用TTC染色法,0.5%TTC溶液,25℃染色24 h;光照12 h条件下以纸床作为发芽床进行发芽试验,试验计数时间为15~45 d。The seed quality inspection method was studied by using the Stemona tuberosa Lour.seeds from Wenshan,Chuxiong and Pu'er of Yunnan Province as materials and referring to GB/T 3543-1995'Inspection Rules for Crop Seeds'.The results showed that the minimum test amount of seed clarity analysis was 75 g,and the sample size was not less than 750 g.The authenticity was determined by the comparison of seed appearance and morphology.The quality was determined by hundred-particle method.The moisture content was determined by drying at high constant temperature(130±2)℃for 5 hours.The seed viability was determined by TTC staining method with 0.5%TTC solution at 25℃for 24 hours.The paper bed was used as the germination bed under the condition of 12 hours light,and the counting time of the test was from 15 to 45 days.

关 键 词：对叶百部 种子 质量检验 

分 类 号：S567.19[农业科学—中草药栽培]