基于双力育种技术筛选铁皮石斛新株系  

Screening of New Dendrobium officinale Strains Based on Dual-capability Breeding Technology

作　　者：吴明开 罗鸣 吴沿胜 吴沿友[2] 陶明清 苏跃 WU Mingkai;LUO Ming;WU Yansheng;WU Yanyou;TAO Mingqing;SU Yue(Guizhou Institute of Crop Germplasm Resources,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China;State Key Laboratory of Environmental Geochemistry,Institute of Geochemistry,Chinese Academy of Sciences,Guiyang 550081,China;Jiangsu Zhongtian Intelligent Sense Life Data Co.,Ltd.,Zhenjiang Jiangsu 212013,China;Engineering Technology Research Center for Protection and Detection of Germplasm Resources of Karst-Adaptable Crops,Guizhou Vocational College of Agriculture,Guiyang 551400,China)

机构地区：[1]贵州省农作物品种资源研究所,贵阳550006 [2]中国科学院地球化学研究所环境地球化学国家重点实验室,贵阳550081 [3]江苏中天智感生命数据有限公司,江苏镇江212013 [4]贵州农业职业学院喀斯特适生作物种质资源保护与检测应用工程技术研究中心,贵阳551400

出　　处：《种子》2024年第9期131-134,共4页Seed

基　　金：贵州省科技计划项目(黔科合服企[2023]007);贵州省科技计划项目(黔科合成果[2022]一般071);贵州省科技计划项目(黔科合支撑[2023]一般363);贵州省科技计划项目(黔科合支撑[2023]一般026);贵州省科技计划项目(黔科合支撑[2020]4Y067号);贵州省中药材现代产业技术体系(GZCYTX2023-0202)。

摘　　要：双力育种技术是利用叶片的电学行为,获取作物的胞内水代谢、物质代谢和能量代谢等植物生理动力学参数,表征作物生命力和生产力(双力)。以“双力”为评价指标在线快速评价作物的生长机能、发育机能、代谢能力和抗逆机能等,对常规育种中的表型和株系、杂交育种的后代以及分子育种的基因型实现在线快速筛选和评价。利用双力育种技术,对16份仿野生附树栽培铁皮石斛资源的叶片胞内水代谢、营养转运能力以及代谢活力进行综合评价,依据得分高低从中筛选出5份适应喀斯特林地附生干燥树皮的铁皮石斛优异株系。继续对得分最高的3份铁皮石斛资源进行筛选和评价,最终获得两次排名均为第一的适宜仿野生干旱逆境铁皮石斛品系LH1,表明双力育种技术能使优良株系筛选效率得到极大的提升。Dual-capability breeding technology uses the electrical behavior of leaves to obtain plant physiological and dynamic parameters such as intracellular water metabolism,material metabolism and energy metabolism of crops to characterize crop vitality and productivity(Dual-capability).The growth function,development function,metabolic ability and stress resistance of crops were rapidly evaluated online with'Dual-capability'as the evaluation index,and the phenotype and strain in conventional breeding,the offspring of cross breeding and the genotype of molecular breeding were quickly screened and evaluated online.The intracellular water metabolism,nutrient transport capacity and metabolic activity of 16 Dendrobium officinale resources were evaluated by dual-capability breeding technique,and 5 excellent Dendrobium officinale lines adapted to the epiphytic dry bark of karst forest were selected according to their scores.The three Dendrobium officinale resources with the highest scores were screened and evaluated,and finally LH1,which ranked first for both times,was suitable for simulating wild drought stress,indicating that dual-capability breeding technology could greatly improve the screening efficiency of excellent strains.

关 键 词：铁皮石斛 双力育种 电生理参数 抗逆机能 优良株系筛选 

分 类 号：S567.239[农业科学—中草药栽培]