蒙古冰草×航道冰草杂种F1代组织培养再生体系建立  

Establishment of Tissue Culture and Regeneration System for F 1 Hybrid of Agropyron mongolicum Keng×Agropyron cristatum cv.Fariway

作　　者：杨东升 卢倩倩 丁伟 王志宇 王志鹏 YANG Dongsheng;LU Qianqian;DING Wei;WANG Zhiyu;WANG Zhipeng(Department of Agronomy,Hetao College,Bayannur Inner Mongolia 015000,China;Key Laboratory of the Development and Resource Utilization of Biological Pesticide atUniversities of Inner Mongolia Autonomous Region,Bayannur Inner Mongolia 015000,China)

机构地区：[1]河套学院农学系,内蒙古巴彦淖尔015000 [2]河套地区绿色农产品安全生产与预警控制内蒙古自治区高等学校重点实验室,内蒙古巴彦淖尔015000

出　　处：《种子》2024年第9期135-140,共6页Seed

基　　金：内蒙古自治区自然科学基金面上项目(2023MS03049);河套学院高层次人才引进科研启动经费资助项目(HYRC202310);河套学院高层次人才引进科研启动经费资助项目(HYRC202310)。

摘　　要：以蒙古冰草×航道冰草种间杂种F1代幼穗为外植体,对影响愈伤组织诱导、分化和生根的激素浓度进行筛选,并建立了完整植株的再生体系。结果表明,杂种F1代的最适愈伤组织诱导培养基为MS+2,4-二氯苯氧乙酸(2,4-D)1.5 mg/L+6-苄氨基嘌呤(6-BA)0.3 mg/L,出愈率为81.7%;愈伤组织分化的培养基为6-BA 2.5 mg/L+萘乙酸(NAA)0.4 mg/L,分化率为71.7%;生根培养基为1/2 MS+NAA 0.2 mg/L,生根率为88.3%。经炼苗后,各基质移栽成活率大于90%。本研究通过建立蒙古冰草×航道冰草杂种F1代组织培养再生体系,为有效保存冰草杂种F1代新种质,并为后期秋水仙碱诱导其染色体加倍恢复育性奠定基础。The F 1 young panicle of the interspecific hybrid of Agropyron mongolicum Keng×Agropyron cristatum cv.Fariway was selected as explants to screen the hormone concentration affecting callus induction,differentiation and rooting,and a complete plant regeneration system was established.The results showed that the optimal callus induction medium of hybrid F 1 generation was MS+2,4-dichlorophenoxyacetic acid(2,4-D)1.5 mg/L+6-benzylaminopurine(6-BA)0.3 mg/L,and the healing rate was 81.7%.The medium for callus differentiation was 6-BA 2.5 mg/L+naphthalene acetic acid(NAA)0.4 mg/L,and the differentiation rate was 71.7%.The rooting medium was 1/2 MS+NAA 0.2 mg/L,and the rooting rate was 88.3%.After refining the seedlings,the survival rate of each substrate transplanting was more than 90%.In this study,the F 1 generation tissue culture regeneration system of Agropyron mongolicum Keng×Agropyron cristatum cv.Fariway hybrid was established to effectively preserve the F 1 generation and laid a foundation for the recovery of chromosome doubling induced by colchicine in the later stage.

关 键 词：蒙古冰草 航道冰草 杂种F1 幼穗 再生体系 

分 类 号：S543.9[农业科学—作物学]