树莓组织培养及快繁体系的建立  

Establishment of Tissue Culture and Rapid Propagation System of Rubus corchorifolius

作　　者：孙杭 申刚 吴小波[1] 王丽敏 HU Yan SUN Hang;SHEN Gang;WANG Limin;WU Xiaobo;HU Yan(Guizhou Institute of Subtropical Crops,Guiyang 560025,China;College of Health Management,Guiyang Healthcare Vocational University,Guiyang 550025,China)

机构地区：[1]贵州省亚热带作物研究所,贵阳560025 [2]贵阳康养职业大学健康管理学院,贵阳550025

出　　处：《种子》2024年第9期141-147,共7页Seed

基　　金：贵州省科技计划项目(黔科合支撑[2021]一般454);贵州省科技计划项目(黔科合服企[2022]008);贵州省黔西南州科技计划课题(2021-1-13);贵州本地特色黑树莓肠道益生酵素饮品开发及产业化关键技术研究(贵康大K2024-22)。

摘　　要：以红树莓、黑树莓、掌叶覆盆子为试验材料,选取茎段、茎尖和叶片作为外植体,建立树莓组织培养快繁体系。结果表明,适宜获得树莓无菌苗的外植体是茎段和茎尖,三种树莓丛生芽诱导最佳培养基为MS+6-BA(1.0~2.0)mg/L+NAA(0.1~0.2)mg/L;最佳愈伤出芽培养基为MS+6-BA(5.0 mg/L)+NAA(0.2 mg/L);红树莓增殖培养的培养基为MS+6-BA(0.5~1.0)mg/L+NAA(0.2 mg/L);黑树莓增殖的培养基为MS+6-BA(1.0 mg/L)+NAA(0.05~0.1)mg/L;掌叶覆盆子增殖的培养基:MS+6-BA(1.0 mg/L)+NAA(0.2 mg/L)。以1/2 MS+IBA(1.0 mg/L)为培养基诱导生根。炼苗在0.05%KMnO4杀菌条件下,在V粘土∶V泥炭土=1∶1的基质中进行。With red Rubus corchorifolius,black Rubus corchorifolius and Rubus chingii Hu as experimental materials,stem segments,stem tips and leaves were selected as explants to establish the rapid propagation system of tissue culture of Rubus corchorifolius.The results showed that the best explants for obtaining the inoculum were stem segment and stem tip,and the best medium for inducing Rubus corchorifolius cluster buds was MS+6-BA(1.0-2.0)mg/L+NAA(0.1-0.2)mg/L.The best callus budding medium was MS+6-BA(5.0 mg/L)+NAA(0.2 mg/L);The medium of red Rubus corchorifolius proliferation culture:MS+6-BA(0.5-1.0)mg/L+NAA(0.2 mg/L);The growth medium of black Rubus corchorifolius was MS+6-BA(1.0 mg/L)+NAA(0.05-0.1)mg/L;The medium for the proliferation of Rubus chingii Hu was MS+6-BA(1.0 mg/L)+NAA(0.2 mg/L).Rooting was induced by 1/2 MS+IBA(1.0 mg/L)medium.The cultivation medium was carried out in V clay to V peat soil=1∶1 under 0.05%KMnO 4 sterilization condition.

关 键 词：树莓 快繁 激素 愈伤组织 

分 类 号：Q943.2[生物学—植物学]