基于代谢组学技术的掌腱膜挛缩症研究  

Metabonomics in dupuytren's disease

作　　者：王震宇 代光明 丰波[1] Wang Zhenyu;Dai Guangming;Feng Bo(Department of Orthopaedic Medicine,the Third Affliated Hospital of Inner Mongolia Medical University,Baotou 014010,China;Department of Hand,Foot,and Ankle Surgery,the Third Affiliated Hospital of Inner Mongolia Medical University,Baotou 014010,China)

机构地区：[1]内蒙古医科大学第三附属医院骨科医学部,包头014010 [2]内蒙古医科大学第三附属医院手足踝外科,包头014010

出　　处：《中华实验外科杂志》2024年第9期2112-2115,共4页Chinese Journal of Experimental Surgery

基　　金：内蒙古自治区自然科学基金项目(2020MS08023)。

摘　　要：目的 利用液相色谱质谱联用(LC/MS)技术探究掌腱膜挛缩症(DD)在代谢组学层面的特性,筛选差异代谢物,为靶标验证提供理论基础.方法 选取2019年1月至2022年12月间在内蒙古包钢医院接受DD手术治疗的患者术中切除的挛缩掌腱膜组织20例为实验组,选取同期行腕管综合征手术治疗的患者术中切取的正常掌腱膜组织20例为对照组.利用LC/MS技术,定性定量分析两组中的代谢物,通过主成分分析及偏最小二乘法判别分析揭示两组代谢差异,进行层次聚类,利用P值、VIP系数及FC值的有效阈值设定筛选出有价值的差异代谢物.绘制箱线图、火山图、和弦图、气泡图、KEGG通路图等观察组间差异及代谢通路富集情况,利用pearson相关系数完成各代谢物的相关性分析,两组间比较采用两独立样本t检验.结果 利用LC/MS技术在40个样本中总计鉴定到931个代谢物,其中439个表达量存在显著差异(P<0.05,VIP>1.00,FC<0.50或FC>2.00).经KEGG通路注释发现,差异代谢物分别在42条通路中富集(x/n>y/N,x:某通路相关差异代谢物数;y:某通路相关总代谢物数;n:经注释的差异代谢物数;N:经注释的总代谢物数).结论 掌腱膜挛缩症在碳代谢、不饱和脂肪酸的生物合成、半胱氨酸和甲硫氨酸代谢三条通路中的异常表现,证明其致病机制与炎症、氧自由基、糖尿病及局部缺血缺氧等因素相关.Objective To explore the metabolic characteristics of Dupuytren's Disease(DD)at the level of metabolomics using liquid chromatograph-mass spectrometer(LC/MS)technology,and to screen for differential metabolites,providing a theoretical basis for target verification.Methods The con-tracted palmar aponeurosis tissue resected during DD surgery in Baogang Hospital of Inner Mongolia from January 2019 to December 2022 was collected as the experimental group,and 20 cases of normal palmar aponeurosis tissue resected during carpal tunnel syndrome surgery in the same period were selected as the control group.Using LC/MS technology,the metabolites in the two groups were analyzed qualitatively and quantitatively,and the metabolic differences between the two groups were revealed by principal component analysis and partial least squares discriminant analysis,hierarchical clustering was performed,and valuable differential metabolites were screened out by the P value,the VIP coefficient,and the effective threshold setting of FC value.Box-and-line diagrams,volcano diagrams,chord diagrams,bubble diagrams,and KEGG pathway diagrams were drawn to observe the intergroup differences and metabolic pathway enrich-ment,and the correlation analysis of each metabolite was completed by Pearson correlation coefficient.T test was used for comparison between the two groups.Results(1)A total of 931 metabolites were iden-tified in 40 samples using LC/MS technology,of which 439 had significantly different expression levels(P<0.05,VIP>1.00,FC<0.50 or FC>2.00).(2)After KEGG pathway annotation,differential me-tabolites were enriched in 42 pathways(x/n>y/N,x:number of pathway-associated differential metabo-lites;y:number of pathway-associated total metabolites;n:number of annotated differential metabolites;N:number of annotated total metabolites).Conclusion Abnormal manifestations of DD in the three path-ways of carbon metabolism,unsaturated fatty acid biosynthesis,and cysteine and methionine metabolism demonstrate that the pathogenic mechanism is rela

关 键 词：掌腱膜挛缩症 代谢组学 液相色谱质谱联用技术 

分 类 号：R658.2[医药卫生—外科学]