探讨形成可用囊胚及优质囊胚相关的胚胎动力学参数  

Exploring embryonic dynamics parameters related to the formation of available and high-quality blastocysts

作　　者：郭小芹 余相 高郁森[1] GUO Xiao-qin;YU Xiang;GAO Yu-sen(Huizhou Second Maternity&Child Healthcare Hospital,Huizhou 516001)

机构地区：[1]惠州市第二妇幼保健院生殖医学中心,惠州516001

出　　处：《生殖医学杂志》2024年第10期1307-1313,共7页Journal of Reproductive Medicine

基　　金：惠州市医疗卫生领域科技计划项目(2022CZ010262)。

摘　　要：目的应用时差成像技术探讨卵裂期胚胎发育参数对形成可用囊胚及优质囊胚的影响。方法回顾性分析2022年9月至2023年9月在我院接受常规IVF治疗的76对不孕不育夫妇的344个胚胎发育及囊胚培养情况。通过时差成像技术,收集记录胚胎的卵裂模式、2细胞时的多核情况、发育至2、3、4、5细胞各阶段的时间,及各胚胎的囊胚培养结果。采用单因素及多因素Logistic分析胚胎卵裂模式、胚胎发育时间参数与可用囊胚/优质囊胚形成的关系。采用回归曲线,绘制2细胞维持时间(T_(3-2))、3细胞维持时间(T_(4-3))、4细胞维持时间(T_(5-4))与可用囊胚率/优质囊胚率的相关图。结果单因素分析结果显示,胚胎异常卵裂、2细胞时多核的出现,以及各个卵裂时间点的延长均会显著影响可用囊胚/优质囊胚的形成(P<0.05)。多因素Logistic分析结果显示,卵裂模式(OR=10.324,P<0.001)、T_(3-2)(OR=1.781,P<0.001)及T_(4-3)(OR=1.492,P=0.029)影响可用囊胚的形成,而卵裂模式(OR=6.546,P=0.001)、T_(4-3)(OR=1.581,P=0.006)及T_(5-4)(OR=1.173,P=0.001)影响优质囊胚的形成。结论卵裂期的非正常卵裂以及T_(3-2)、T_(4-3)、T_(5-4)处于优势时间外的胚胎,其可用囊胚及优质囊胚形成率均会下降。Objective:To explore the development parameters of cleavage embryos related to the formation of available and high-quality blastocysts by using time-lapse imaging technology.Methods:A retrospective analysis was conducted on the development and blastocyst culture of 344 embryos in 76 infertile couples who underwent conventional IVF treatment in Huizhou Second Maternity&Child Healthcare Hospital during September 2022 to September 2023.The time-lapse imaging culture system was used to collect and record the cleavage pattern of embryos,the multi-nucleation at 2-cell stage,the cleavage time of 2-,3-,4-,and 5-cell stage,as well as the blastocyst culture results of each embryo.The univariate and multivariate logistic analyses were used to evaluate the relationship between embryo cleavage pattern,time parameters,and the formation of available and high-quality blastocyst.The regression curves were drawn for the correlation between the length of the second(T_(3-2)),third(T_(4-3)),forth(T_(5-4))cell cycle and the rates of available and high-quality blastocyst.Results:The univariate analysis showed that abnormal cleavage patterns,multi-nucleation at 2-cell stage,and the delay of different cleavage time had significant influences on the formation of available and high-quality blastocyst.The multivariate logistic analysis found that cleavage patterns(OR=10.324,P<0.001)as well as T_(3-2)(OR=1.781,P<0.001)and T_(4-3)(OR=1.492,P=0.029)were associated with the formation of available blastocysts.And cleavage patterns(OR=6.546,P=0.001),T_(4-3)(OR=1.581,P=0.006),T_(5-4)(OR=1.173,P=0.001)had impact on the formation of high-quality blastocysts.Conclusions:Abnormal cleavage pattern,as well as embryos outside the dominant time of T_(3-2),T_(4-3),and T_(5-4),could result in a decreased rate in the formation of available and high-quality blastocysts.

关 键 词：时差成像 卵裂模式 时间参数 

分 类 号：R711.6[医药卫生—妇产科学]