Employing pH-responsive RNA triplex to control CRISPR/Cas9-mediated gene manipulation in mammalian cells  

作　　者：Yan Liu Yang Wang Jiayi Zhu Xuxian Su Xudong Lin Liang Xu Xiwen Xing 

机构地区：[1]Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument,School of Biomedical Engineering,Shenzhen Campus of Sun Yat-sen University,Sun Yat-sen University,Shenzhen 518107,China [2]MOE Key Laboratory of Bioinorganic and Synthetic Chemistry,School of Chemistry,Sun Yat-sen University,Guangzhou 510275,China [3]Department of Cardiology,the first people’s hospital of Foshan,Foshan 528000,China [4]Department of Biotechnology,College of Life Science and Technology,Jinan University,Guangzhou 510632,China

出　　处：《Chinese Chemical Letters》2024年第9期219-222,共4页中国化学快报（英文版）

基　　金：supported by the National Key R&D Program of China(Nos.2022YFC2804101,2020YFA0211200);National Natural Science Foundation of China(Nos.22377056,22222706,21977122)。

摘　　要：The clustered regularly interspersed short palindromic repeats/CRISPR-associated protein 9(CRISPR/Cas9)system is an RNA-guided platform for highly efficient and specific genome targeting in diverse organisms,which has been exploited for various applications in gene manipulation.Compared with the constantly active CRISPR/Cas9 function,conditional control of its activity can improve the performance of the system with reduced side effects and high spatiotemporal precision.The pH-responsive triplex RNA was successful used in CRISPR-derived RNA/trans-activating crRNA(crRNA/tracrRNA)of CRISPR/Cas9,thus affecting RNA/dead Cas9(dCas9)complex to target DNA in vitro and in vivo.This design of triplex RNA opens a new window towards the broad involvement of eukaryotic cells for conditional control of CRISPR/Cas9function.?2024 Published by Elsevier B.V.on behalf of Chinese Chemical Society and Institute of Materia Medica,Chinese Academy of Medical Sciences.

关 键 词：RNA triplex PH-RESPONSIVE CRISPR/Cas9 Gene manipulation Eukaryotic cells 

分 类 号：Q78[生物学—分子生物学]