Preparation of norovirus GII loop mediated isothermal amplification freeze-drying microsphere reagents and its application in an on-site integrated rapid detection platform  

作　　者：Yanqi Wu Yuhong Guan Peilin Huang Hui Chen Liping Bai Zhihong Jiang 

机构地区：[1]State Key Laboratory of Quality Research in Chinese Medicine,Macao University of Science and Technology,Macao 999078,China [2]Shenzhen LemnisCare Medical Technology Co.,Ltd.,Shenzhen 518107,China [3]Hunan Key Laboratory of Biomedical Nanomaterials and Devices,Hunan University of Technology,Zhuzhou 412077,China

出　　处：《Chinese Chemical Letters》2024年第9期262-265,共4页中国化学快报（英文版）

基　　金：funded by the Science and Technology Development Fund,Macao SAR(Nos.0065/2020/A2,SKLQRCM(MUST)-2020-2022);Shenzhen-Hong Kong-Macao Science and Technology Project(Grade c)(No.SGDX20210823104201010).

摘　　要：Norovirus is an infectious disease that can cause non-bacterial gastroenteritis,which has a low infectious dose,rapid onset,and strong transmission ability;therefore,rapid and sensitive detection is essential to reduce the transmission of gastroenteritis.In the study,a norovirus GII loop-mediated isothermal amplification assay was developed and prepared into freeze-drying microspheres,and a closed-cassette-based,integrated,reagent-ambient storage,on-site instant detection platform for norovirus GII was constructed using a commercial,fully automated nucleic acid analyzer with integrated magnetic bearing based nuclear acid extraction and nucleic acid detection,with a sensitivity of 10 copies/μL,with no cross-reactivity with other 5 viruses.For 28 simulated samples,the integrated assay platform was consistent with the experimental results of reverse transcription-quantitative polymerase chain reaction(RT-qPCR)assays after conventional laboratory nucleic acid extraction.The entire process can be finished in about 1 h,which is ideal for immediate rapid detection.

关 键 词：Freeze-dried microspheres Point-of-care testing Loop-mediated isothermal amplification Norovirus GII Integrated testing 

分 类 号：R943[医药卫生—药剂学]