Colorimetric detection of α-glucosidase activity using Ni-CeO_(2)nanorods and its application to potential natural inhibitor screening  

作　　者：Jia Chen Yun Liu Zerong Long Yan Li Hongdeng Qiu 

机构地区：[1]Xinjiang Uygur Autonomous Product Quality Supervision and Inspection Institute,Urumqi 830000,China [2]CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province,Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,Lanzhou 730000,China

出　　处：《Chinese Chemical Letters》2024年第9期387-391,共5页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.22374159);Regional Collaborative Innovation Project of Xinjiang(No.2020E01051);CAS-JSPS Cooperation and Exchange Program(No.E20489GHZ1);the Youth Innovation Promotion Association CAS(No.2021420)。

摘　　要：In this work,we established an exceptionally facile method for the preparation of Ni-CeO_(2)nanorods in a kind of deep eutectic solvents(DESs)composed of l-proline and Ce(NO_(3))_(3)·6H_(2)O.First,Ni-CeO_(2)nanorods were successfully prepared by adding Ni(NO_(3))_(3)·6H_(2)O to DESs.Then,we found that Ni-CeO_(2)nanorods prepared in DESs have more prominent oxidase-like activity than pure CeO_(2).The outstanding catalytic activity of Ni-CeO_(2)could be ascribed to its high Ce^(3+)/Ce^(4+)ratio.As a proof-of-concept application,the Ni-CeO_(2)nanorods were successfully acted as a colorimetric platform for the sensitive determination of ascorbic acid andα-glucosidase activity,which displays excellent analytical performance.Moreover,this sensing platform was applied for screening naturalα-glucosidase inhibitors,such as terpenoids from natural products.The results indicated that ursolic acid and oleanolic acid had good inhibitory rates.This strategy not only provides a new way to construct more kinds of nanomaterials from DESs,but also offers a facile and effective tool to screen theα-glucosidase natural inhibitors as potential anti-diabetic drugs.

关 键 词：Ni-CeO_(2) Ascorbic acid Α-GLUCOSIDASE Inhibitor screening Nanozyme 

分 类 号：TQ460.1[化学工程—制药化工] TB383.1[一般工业技术—材料科学与工程] O657[理学—分析化学]