壳聚糖/k-卡拉胶寡糖温敏水凝胶的制备与表征  

Synthesis and Analysis of Thermosensitive Hydrogels from Chitosan/k-Carrageenan Oligosaccharides

作　　者：李岩峰 王勇[1] 白桐宁 董硕[1] 王康康 孙昊鉴 吴海歌[1] 姚子昂 LI Yan-feng;WANG Yong;BAI Tong-ning;DONG Shuo;WANG Kang-kang;SUN Hao-jian;WU Hai-ge;YAO Zi-ang(College of Life and Health,Dalian University,Dalian 116622,China;College of Life Sciences,Dalian Nationalities University,Dalian 116600,China)

机构地区：[1]大连大学生命健康学院,大连116622 [2]大连民族大学生命科学学院,大连116600

出　　处：《高分子通报》2024年第10期1428-1437,共10页Polymer Bulletin

基　　金：辽宁省教育厅科学研究项目(项目号jyt-dldxjc202002)。

摘　　要：利用k-卡拉胶寡糖(KOS)和壳聚糖(CS)带有不同电荷的特点,以KOS为交联剂,通过离子交联制备可以缓释KOS和可控降解的温敏性水凝胶。将CS酸性溶液与KOS水溶液在室温下混合,制备KOS与CS质量比为2.25:1.0~2.75:1.0的系列凝胶体系。使用试管倒置法测凝胶时间,差值法测相变体积变化,称重法测溶胀度和降解率,苯酚-硫酸法测凝胶对KOS的缓释,在电子扫描显微镜(SEM)下观察凝胶表面结构,MTT法检测中枢神经免疫效应细胞(N9)在凝胶上的粘附和增殖情况。结果表明KOS/CS系列水凝胶具有温度响应性,在4℃呈现为液态的溶胶状态,在生理温度条件下(37℃)可相变为固形水凝胶,凝胶时间为(300±50)~(17±5)min。凝胶后具有明显的相变体积变化,孔隙率为(78.7±2.5)%~(92.9±2.1)%,溶胀率为(558.7±5.046)%~(853.9±6.143)%,降解率为(77.46±3.520)%~(70.68±4.080)%以及KOS缓释程度为(30.38±0.9523)%~(24.24±3.988)%;该凝胶在SEM下呈纤维多孔结构,且支持N9细胞的黏附和增殖。KOS/CS水凝胶具有良好的温敏性、溶胀性、可控降解性、KOS缓释能力和细胞相容性,可为药物递送和组织工程领域提供较好的生物材料。Taking advantage of the different charges of k-carrageenan oligosaccharide(KOS)and chitosan(CS),using KOS as a cross-linking agent,a thermosensitive hydrogel which can release KOS and control degradation can be prepared by ionic cross-linking glue.The CS acid solution and the KOS aqueous solution are mixed at room temperature to prepare a series of gel systems with the mass ratio of KOS to CS ranging from 2.25:1.0 to 2.75:1.0.The gel time is determined by the test tube inversion method,the volume change of phase transition is measured by the difference method,the swelling degree and degradation rate are measured by the weighing method,the sustained release of KOS from the gel is measured by the phenol-sulfuric acid method,and gel surface structure is observed under a scanning electron microscope(SEM).The adhesion and proliferation of central nervous immune effector cells(N9)on the gel were detected by MTT method.The results show that a series of KOS/CS hydrogels are temperature-responsive,appearing in a liquid sol state at 4°C,and can be phase-transformed into solid hydrogels at physiological temperature(37°C),and the gel time is 300±50–17±5 min.After gelation,there is obvious phase transition volume change,the porosity is(78.7±2.5)%–(92.9±2.1)%,the swelling rate is(558.7±5.046)%–(853.9±6.143)%,the degradation rate is(77.46±3.520)%–(70.68±4.080)%and the KOS sustained release degree is(30.38±0.9523)%–(24.24±3.988)%;the gel shows a fibrous porous structure under SEM,and supports the adhesion and proliferation of N9 cells.KOS/CS hydrogel has good temperature sensitivity,swelling property,controllable degradability,sustained release ability for KOS and cytocompatibility,which can provide a better biomaterial for the fields of drug delivery and tissue engineering.

关 键 词：壳聚糖 k-卡拉胶寡糖 水凝胶 生物相容性 

分 类 号：TQ427.26[化学工程]