舟山市HIV/AIDS病例HIV-1分子传播网络分析  

HIV-1 molecular transmission network among HIV/AIDS cases in Zhoushan City

作　　者：陈圆静 李科峰[1] 范钦[2] 张佳峰[2] 吴铭宇[1] 张波 顾松叶[1] 王海[1] 程伟[2] CHEN Yuanjing;LI Kefeng;FAN Qin;ZHANG Jiafeng;WU Mingyu;ZHANG Bo;GU Songye;WANG Hai;CHENG Wei(Department of HIV/AIDS and TB Control and Prevention,Zhoushan Center for Disease Control and Prevention,Zhoushan,Zhejiang 316021,China;Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou,Zhejiang 310051,China;Dinghai District Center for Disease Control and Prevention,Zhoushan,Zhejiang 316000,China)

机构地区：[1]舟山市疾病预防控制中心艾滋病结核病防制科,浙江舟山316021 [2]浙江省疾病预防控制中心,浙江杭州310051 [3]舟山市定海区疾病预防控制中心,浙江舟山316000

出　　处：《预防医学》2024年第10期834-837,共4页China Preventive Medicine Journal

基　　金：浙江省科技计划项目(2024C35088);舟山市医药卫生科技计划项目(2023YA12)。

摘　　要：目的了解浙江省舟山市艾滋病病毒感染者和艾滋病患者(HIV/AIDS)艾滋病病毒1型(HIV-1)分子传播网络特征。方法选择2020—2022年舟山市新报告的HIV/AIDS病例为研究对象,首次随访时收集基本信息并采集全血标本,采用反转录PCR和巢式PCR扩增HIV-1 pol区序列,构建Neighbor-Joining系统进化树鉴定HIV-1亚型,采用Cytoscape 3.6.1软件构建HIV-1分子传播网络进行分析。结果2020—2022年舟山市新报告HIV/AIDS病例222例,收集全血样本200例,检测获得合格序列152例,其中男性122例,占80.26%;年龄≥50岁75例,占49.34%;初中及以下文化程度109例,占71.71%;经商业异性性行为传播63例,占41.45%。检出HIV-1亚型以CRF07_BC和CRF01_AE为主,分别占45.39%和21.05%。基因距离阈值设为1%时,有69例HIV/AIDS病例形成20个分子簇,成簇率为45.39%;以2020年报告的HIV/AIDS病例构成的分子网络为对照,2022年新增病例数≥5例的活跃分子簇有2个,各9例病例,以年龄≥50岁、初中及以下文化程度和经商业异性性性行为传播为主要特征。结论舟山市HIV/AIDS病例HIV-1优势型为CRF07_BC和CRF01_AE,中老年人商业异性性行为传播是主要传播模式。Objective To investigate the characteristics of HIV-1 molecular transmission network among HIV/AIDS pa⁃tients in Zhoushan City,Zhejing Province.Methods The newly reported HIV/AIDS cases in Zhoushan City from 2020 to 2022 were selected.Basic information was collected and whole blood samples were obtained at the initial follow-up.The pol gene sequences of HIV-1 were amplified by RT-PCR and nested-PCR.HIV-1 subtypes were identified by Neighbor-Joining phylogenetic trees.The HIV-1 molecular transmission network was built and analyzed using Cytoscape 3.6.1 software.Results A total of 222 HIV/AIDS cases were reported in Zhoushan City from 2020 to 2022,200 whole blood samples were collected,and 152 sequences were obtained successfully,including 122 males(80.26%),75 cases aged 50 years and above(49.34%),109 cases with a junior high school education or below(71.71%),and 63 cases with commercial heterosexual contact(41.45%).The main subtypes were CRF07_BC and CRF01_AE,accounting for 45.39%and 21.05%,respectively.When the threshold of genetic distance was set to 1%,20 molecular clusters were formed in 69 cases,with a clustering rate of 45.39%.Using the molecular network constituted by reported HIV/AIDS cases in 2020 as the baseline network,there were 2 active molecular clusters with≥5 new cases in 2022,each with 9 cases,characterized mainly by individuals aged 50 or above,with a junior high school education or below,and transmis⁃sion through commercial heterosexual sex.Conclusion The predominant HIV-1 subtypes among HIV/AIDS cases in Zhoushan City are CRF07_BC and CRF01_AE.Transmission through commercial heterosexual contact among middle�aged and elderly people is a main mode of HIV transmission.

关 键 词：艾滋病病毒 分子传播网络 分子簇 

分 类 号：R512.91[医药卫生—内科学]