转抗虫耐除草剂基因玉米对根际细菌群落结构和功能的影响  

Effects of Transgenic Insect-Resistant and Herbicide-Tolerant Genes Maize on Rhizosphere Bacterial Community Structure and Function

作　　者：郑怀谷 胡刚 于赐刚 章嫡妮 王琛[1] 张振华 ZHENG Huaigu;HU Gang;YU Cigang;ZHANG Dini;WANG Chen;ZHANG Zhenhua(School of Earth Sciences,Zhejiang University,Hangzhou 310058,China;Nanjing Institute of Environmental Sciences,MEE,Nanjing 210042,China;School of Engineering,China Pharmaceutical University,Nanjing 211198,China)

机构地区：[1]浙江大学地球科学学院,浙江杭州310058 [2]生态环境部南京环境科学研究所,江苏南京210042 [3]中国药科大学工学院,江苏南京211198

出　　处：《环境科学研究》2024年第10期2171-2180,共10页Research of Environmental Sciences

基　　金：生态环境部生物多样性调查、观测和评估项目(2019-2023年)。

摘　　要：抗虫耐除草剂是转基因玉米的最主要性状,根际土壤细菌是转基因植物生态风险评估中的重要监测对象,目前关于转抗虫耐除草剂基因对玉米根际土壤细菌群落结构和功能的影响尚不清楚。本研究以华兴单和罗单2个转基因玉米品种及其亲本为研究对象,采用高通量测序技术并结合PICRUSt功能预测,分析转基因对玉米根际细菌群落结构和功能的影响。结果表明:①转基因对细菌主要门和属的组成及丰度没有显著影响,但对硝化螺菌门、鞘氨醇单胞菌属和硝化螺菌属等丰度有显著影响。②冗余分析表明,土壤pH、电导率及速效磷对细菌群落结构具有显著影响,但转基因的影响并不显著。③PICRUSt功能预测结果表明,转基因对31种二级功能没有显著影响,对氮磷循环功能影响也不明显;而玉米品种和生长阶段对25种二级功能具有显著影响,而且品种也是影响氮磷循环相关功能的重要因素。研究显示,转基因对玉米根际细菌群落结构及功能并无显著影响,而玉米品种和生长阶段的影响则更为显著。Insect-resistant and herbicide-tolerant are the primary traits of transgenic maize,and rhizosphere bacteria are important monitoring targets for the ecological risk assessment of transgenic plants.The effects of transgenic insect-resistant and herbicide-tolerant genes on the structure and function of maize rhizosphere bacterial communities remains unclear.This study focused on two transgenic maize lines(HX and LD)and their parental lines,and analyzed the effects of transgenic trait on the structure and function of maize rhizosphere bacterial communities using high-throughput sequencing technology combined with PICRUSt function prediction.The results showed that:(1)The transgenic trait had no significant effect on the composition and abundance of major bacterial phyla and genera,but significantly impacted the abundance of Nitrospiraea and certain bacterial genera such as Sphingomonas and Nitrospira.(2)Redundancy analysis revealed that soil pH,EC and available phosphorus significantly affected bacterial community structure,while the effect of the transgenic trait was not significant.(3)PICRUSt function prediction indicated that the transgenic trait had no significant effect on 31 secondary functions,including nitrogen and phosphorus cycling-related functions.Conversely,the maize line and growth stage significantly affected 25 secondary functions,with the line being an important factor influencing nitrogen and phosphorus cycling-related functions.The above results indicate that transgenic traits have no significant effect on the structure and function of maize rhizosphere bacterial community,while the effects of maize line and growth stage are more significant.

关 键 词：转抗虫耐除草玉米 品种 根际细菌群落 PICRUSt 氮磷循环 

分 类 号：X83[环境科学与工程—环境工程]