负载水飞蓟宾的UCNP-mSiO_(2)的合成、表征及其抗肿瘤性能  

Synthesis and Characterisation of UCNP-mSiO_(2) Loaded with Silymarin and Its Anti-tumor Properties

作　　者：程亚青 刘爱萍 李佟 张仁文 葛雅琨 张元新 CHENG Ya-qing;LIU Ai-ping;LI Tong;ZHANG Ren-wen;GE Ya-kun;ZHANG Yuan-xin(College of Biology and Food Engineering,Jilin Institute of Chemical Technology,Jilin 132022,China)

机构地区：[1]吉林化工学院生物与食品工程学院,吉林吉林132022

出　　处：《化学试剂》2024年第10期7-13,共7页Chemical Reagents

基　　金：吉林省科技发展计划项目(20210204026YY)。

摘　　要：研究设计一种用于乳腺癌治疗的缓释水飞蓟宾的上转换介孔二氧化硅纳米复合体系。首先采用高温溶剂热法制备NaYF4∶Yb,Er(UCNP)纳米颗粒,并在纳米颗粒表面包裹介孔二氧化硅(UCNP@mSiO_(2)),其次在UCNP-mSiO_(2)中负载药物水飞蓟宾(INN);本研究利用X射线衍射仪、高分辨率透射电子显微镜对纳米颗粒进行结构、形貌的表征,并检测其载药性能和体外释放能力;通过人乳腺癌细胞(MCF-7)检测该纳米颗粒的生物安全性及杀伤效果;采用蛋白免疫印迹实验检测UCNP@mSiO_(2)-INN诱导MCF-7细胞凋亡发生的可能机制。实验结果表明,成功合成了UCNP@mSiO_(2)-INN纳米颗粒,具有较强的生物安全性和肿瘤杀伤能力,UCNP@mSiO_(2)-INN以剂量依赖方式抑制MCF-7细胞增殖,其机制可能是通过线粒体凋亡途径实现的。研究结果为药物的开发和利用提供了新思路。The upconverted mesoporous silica nanocomposite system of slow-release silymarin for breast cancer therapy was designed.Firstly,we prepared NaYF 4∶Yb,Er(UCNP)nanoparticles by high temperature solvothermal method,and then encapsulated mesoporous silica on the surface of nanoparticles(UCNP@mSiO_(2)),consequently loading the drug silymarin(INN)into UCNP-mSiO_(2).The structure and morphology of nanoparticles were characterized by using X-ray diffractometry and high-resolution transmission electron microscope.The drug-carrying property and in vitro release ability were also examined.The biosafety and killing effect of nanoparticles were examined by human breast cancer cells(MCF-7).The protein immunoblotting experiments were used to examine the possible mechanism of the occurrence of apoptosis induced by UCNP@mSiO_(2)-INN in MCF-7 cells.The results showed that UCNP@mSiO_(2)-INN nanoparticles were successfully synthesized with strong biosafety and tumor-killing ability.UCNP@mSiO_(2)-INN inhibited the value-added of MCF-7 cells in a dose-dependent manner by a mechanism that may be achieved through the mitochondrial apoptosis pathway.The research provided a new idea for drug development and utilization.

关 键 词：上转换纳米材料 介孔二氧化硅 水飞蓟宾 乳腺癌 细胞凋亡 

分 类 号：R285[医药卫生—中药学]