基于UPLC-Q-TOF-MS/MS技术的灰茅根化学成分分析  

Analysis of Chemical Constituents in Root Pennisetum by High Resolution UPLC-Q-TOF-MS/MS

作　　者：于亚娟 周轲 刘东升 朱旭江 郭朝晖 袁松[3] 石晶 YU Ya-juan;ZHOU Ke;LIU Dong-sheng;ZHU Xu-jiang;GUO Zhao-hui;YUAN Song;SHI Jing(College of Pharmacy,Gansu University of Traditional Chinese Medicine,Lanzhou 730000,China;Key Laboratory of Quality Control of Chinese Herbal Medicine Slices,State Drug Administration,Gansu Institute of Drug Inspection,Lanzhou 730000,China;China National Institute for Food and Drug Control,Beijing 100004,China)

机构地区：[1]甘肃中医药大学药学院,甘肃兰州730000 [2]甘肃省药品检验研究院国家药品监督管理局中药材饮片质量控制重点实验室,甘肃兰州730000 [3]中国食品药品检定研究院,北京100004

出　　处：《化学试剂》2024年第10期63-71,共9页Chemical Reagents

基　　金：兰州市科技计划项目(2019-1-127);甘肃省药品科研项目(2018GSFDA004、SSCJG-YP-A202201、2022GSMPA0026、2022GSMPA0032)。

摘　　要：采用超高效液相色谱串联四级杆飞行时间质谱(UPLC-Q-TOF-MS/MS)技术对灰茅根中化学成分进行定性分析。采用Waters ACQUITY UPLC BEH C 18色谱柱,以0.1%甲酸水-乙腈为流动相设置梯度洗脱,流速为0.3 mL/min。质谱检测后得到多个不同的化合物,比较提取物中分子量和碎片分子量,并借助数据库搜索。共鉴定出41个化合物,其中萜类10个,苯丙素类9个,黄酮类5个,醌类2个,有机酸4个,生物碱2个和其他类7个。利用此法能有效、快速地检测出灰茅根中的化学成分,为中药材来源的先导化合物的开发提供理论指导。UPLC-Q-TOF-MS/MS was used to qualitatively analyze the chemical components of root Pennisetum.The chromatographic column was Waters ACQUITY UPLC BEH C 18,with a gradient elution of 0.1%formic acid water-acetonitrile as the mobile phase and the flow rate was 0.3 mL/min.Several compounds were identified using mass spectrometry,and the molecular weights of the extracts and fragments were compared by searching against a database.A total of 41 chemical compounds were identified,including 10 terpenes,9 phenylpropanoids,5 flavonoids,2 quinones,4 organic acids,2 alkaloids and 7 others.This method can effectively and quickly detect the chemical constituents in roots of Pennisetum,and provide theoretical guidance for the development of lead compounds from Chinese medicinal materials.

关 键 词：UPLC-Q-TOF-MS/MS 灰茅根 化学成分 萜类化合物 苯醇类化合物 

分 类 号：R917[医药卫生—药物分析学]