Effect of cuproptosis on acute kidney injury after cardiopulmonary bypass in diabetic patients  

作　　者：Xi-Jin Deng Yi-Nan Wang Chuan-Bao Lv Zhong-Zhi Qiu Ling-Xin Zhu Jing-Hui Shi Si-Ri-Gu-Leng Sana 

机构地区：[1]Department of Anesthesiology,The Second Affiliated Hospital of Harbin Medical University,Harbin 150001,Heilongjiang Province,China [2]Department of The Health Management Service Evaluation Center,The Health Management Service Evaluation Center of Heilongjiang Province,Harbin 150000,Heilongjiang Province,China [3]Department of Anesthesiology,The First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 150001,Guangdong Province,China [4]Department of Anesthesiology,The First Affiliated Hospital of Harbin Medical University,Harbin 150001,Heilongjiang Province,China [5]Department of Thoracic Surgery,The First Affiliated Hospital of Harbin Medical University,Harbin 150001,Heilongjiang Province,China

出　　处：《World Journal of Diabetes》2024年第10期2123-2134,共12页世界糖尿病杂志（英文）

基　　金：Supported by the Natural Science Foundation of Heilongjiang Province,No.LH2022H035;the First Affiliated Hospital of Harbin Medical University Foundation,No.2024JQ14.

摘　　要：BACKGROUND Cardiopulmonary bypass(CPB)is a common procedure in cardiac surgery.CPB is a high-risk factor for acute kidney injury(AKI),and diabetes is also such a factor.Diabetes can lead to copper overload.It is currently unclear whether AKI after CPB in diabetic patients is related to copper overload.AIM To explore whether the occurrence of CPB-AKI in diabetic patients is associated with cuproptosis.METHODS Blood and urine were collected from clinical diabetic and non-diabetic patients before and after CPB.Levels of copper ion,lactate,glucose,heat shock protein-70(HSP-70),and dihydrolipoamide dehydrogenase(DLAT)were determined.A diabetic rat model was established and CPB was performed.The rats were assessed for the development of CPB-AKI,and for the association of AKI with cuproptosis by detecting copper levels,iron-sulfur cluster proteins and observation of mitochondrial structure by electron microscopy.RESULTS CPB resulted in elevations of copper,lactate,HSP-70 and DLAT in blood and urine in both diabetic and nondiabetic patients.CPB was associated with pathologic and mitochondrial damage in the kidneys of diabetic rats.Cuproptosis-related proteins also appeared to be significantly reduced.CONCLUSION CPB-AKI is associated with cuproptosis.Diabetes mellitus is an important factor aggravating CPB-AKI and cuproptosis.

关 键 词：Cardiopulmonary bypass Acute kidney injury Cuproptosis DIABETES Copper overload Iron-sulfur cluster proteins 

分 类 号：R587.1[医药卫生—内分泌]