基于Gabor模式的多光谱数字全息显微成像系统  

Multispectral Microimaging System Based on Gabor In-line Digital Holography

作　　者：李心语 冯飞 孟浩然 崔旭 时璐 杨萱 杨海贵 Li Xinyu;Feng Fei;Meng Haoran;Cui Xu;Shi Lu;Yang Xuan;Yang Haigui(Key Laboratory of Optical System Advanced Manufacturing Technology,Changchun Institute of Optics,Fine Mechanics and Physics,Chinese Academy of Sciences,Changchun 130033,Jilin,China;University of Chinese Academy of Sciences,Beijing 100049,China;Beijing Institute of Tracking and Telecommunication Technology,Beijing 100094,China)

机构地区：[1]中国科学院长春光学精密机械与物理研究所光学系统先进制造技术重点实验室,吉林长春130033 [2]中国科学院大学,北京100049 [3]北京跟踪与通信技术研究所,北京100094

出　　处：《激光与光电子学进展》2024年第16期242-250,共9页Laser & Optoelectronics Progress

基　　金：国家重点研发计划项目(2021YFC3101300)。

摘　　要：为了同时对目标样本的三维空间结构及光谱信息进行分析,提出了基于Gabor模式的多光谱数字全息显微成像系统。即在Gabor同轴数字全息成像系统中引入可调谐多波段LED照明光源,获得目标样本在不同波长下的全息图像,通过采用角谱自聚焦和孪生像抑制算法获得目标样本在不同波长下的重建图像,估计样本透射光谱,实现像素级图像配准。使用美国空军(USAF)分辨率板对系统成像性能进行测试,选取不同生物组织样本及不同属性液体进行多光谱实验探究。结果表明,该系统成像分辨率可达7.81μm,不仅实现了彩色全息显微成像,具有良好的色彩再现像,还可以定性区分不同属性液体,在无标记生物样品的成像和检测领域中具有潜在的应用前景。To achieve simultaneous analysis of the three-dimensional spatial structure and spectral information of a target sample,a multi-spectral Gabor in-line digital holographic microscopy imaging system is proposed.Specifically,an adjustable multi-wavelength LED illumination source is introduced into the Gabor coaxial digital holographic imaging system.Holographic images of a target sample at different wavelengths are acquired,and the reconstructed images of the target sample at various wavelengths are obtained through angular spectrum autofocusing and twin-image suppression algorithms.This allows the estimation of the sample’s transmission spectra and realizes pixel-level image registration.The imaging performance of the system is tested using the United States air force(USAF)resolution chart,and multispectral experiments are conducted considering different biological tissue samples and liquids with different properties.The results demonstrate that the system can achieve an imaging resolution of up to 7.81μm.Furthermore,the proposed imaging technique not only achieves color holographic microscopy imaging with good color reproduction,but also qualitatively distinguish liquids with different properties,which has potential application prospects in the imaging and detection of unlabeled biological samples.

关 键 词：成像系统 全息重建 多光谱成像 显微成像 Gabor数字全息 

分 类 号：O438.1[机械工程—光学工程]