Stimulation and imaging of neural cells via photonic nanojets  

作　　者：HENG LI XIXI CHEN TIANLI WU ZHIYONG GONG JINGHUI GUO XIAOSONG BAI JIAWEI LI YAO ZHANG YUCHAO LI BAOJUN LI 

机构地区：[1]Guangdong Provincial Key Laboratory of Nanophotonic Manipulation,Institute of Nanophotonics,College of Physics&Optoelectronic Engineering,Jinan University,Guangzhou 511443,China [2]School of Medicine,The Chinese University of Hong Kong,Shenzhen 518172,China [3]InnerMedical Co.,Ltd.,Shenzhen 518055,China

出　　处：《Photonics Research》2024年第8期1604-1609,共6页光子学研究（英文版）

基　　金：National Natural Science Foundation of China(62135005,12304322,62305132);Guangdong Basic and Applied Basic Research Foundation(2021B1515020046)。

摘　　要：Various neuromodulation techniques have been developed to modulate the peak activity of neurons,thereby regulating brain function and alleviating neurological disorders.Additionally,neuronal stimulation and imaging have significantly contributed to the understanding and treatment of these diseases.Here,we propose utilizing photonic nanojets for optical stimulation and imaging of neural cells.The application of resin microspheres as microlenses enhances fluorescence imaging of neural lysosomes,mitochondria,and actin filaments by generating photonic nanojets.Moreover,optical tweezers can precisely manipulate the microlenses to locate specific targets within the cell for real-time stimulation and imaging.The focusing capabilities of these microlenses enable subcellular-level spatial precision in stimulation,allowing highly accurate targeting of neural cells while minimizing off-target effects.Furthermore,fluorescent signals during neural cell stimulation can be detected in real-time using these microlenses.The proposed method facilitates investigation into intercellular signal transmission among neural cells,providing new insights into the underlying mechanisms of neuronal cell activities at a subcellular level.

关 键 词：STIMULATION NEURAL utilizing 

分 类 号：O439[机械工程—光学工程] Q189[理学—光学]