Fourier-domain-compressed optical time-stretch quantitative phase imaging flow cytometry  

作　　者：RUBING LI YUEYUN WENG SHUBIN WEI SIYUAN LIN JIN HUANG CONGKUAN SONG HUI SHEN JINXUAN HOU YU XU LIYE MEI DU WANG YUJIE ZOU TAILANG YIN FULING ZHOU QING GENG SHENG LIU CHENG LEI 

机构地区：[1]Institute of Technological Sciences,Wuhan University,Wuhan 430072,China [2]School of Power and Mechanical Engineering,Wuhan University,Wuhan 430072,China [3]Department of Thoracic Surgery,Renmin Hospital,Wuhan University,Wuhan 430060,China [4]Department of Hematology,Zhongnan Hospital,Wuhan University,Wuhan 430071,China [5]Department of Thyroid and Breast Surgery,Zhongnan Hospital,Wuhan University,Wuhan 430071,China [6]Department of Radiation and Medical Oncology,Zhongnan Hospital,Wuhan University,Wuhan 430071,China [7]School of Computer Science,Hubei University of Technology,Wuhan 430068,China [8]Reproductive Medical Center,Renmin Hospital,Wuhan University,Wuhan 430060,China [9]Suzhou Institute of Wuhan University,Suzhou 215000,China [10]Shenzhen Institute of Wuhan University,Shenzhen 518057,China

出　　处：《Photonics Research》2024年第8期1627-1639,共13页光子学研究（英文版）

基　　金：National Key Research and Development Program of China(2023YFF0723300);Fundamental Research Funds for the Central Universities(2042023kf0105,2042024kf0003,2042024kf1010);Science Fund for Distinguished Young Scholars of Hubei Province(2021CFA042);Natural Science Foundation of Hubei Province(2023AFB133);National Natural Science Foundation of China(12374295,62075200);Interdisciplinary Innovative Talents Foundation from Renmin Hospital of Wuhan University(JCRCYR-2022-006);Jiangsu Science and Technology Program(BK20221257);Shenzhen Science and Technology Program(JCYJ20220530140601003,JCYJ20230807090207014);Translational Medicine and Multidisciplinary Research Project of Zhongnan Hospital of Wuhan University(ZNJC202217)。

摘　　要：Optical time-stretch(OTS)imaging flow cytometry offers a promising solution for high-throughput and highprecision cell analysis due to its capabilities of high-speed,high-quality,and continuous imaging.Compressed sensing(CS)makes it practically applicable by significantly reducing the data volume while maintaining its highspeed and high-quality imaging properties.To enrich the information of the images acquired with CS-equipped OTS imaging flow cytometry,in this work we propose and experimentally demonstrate Fourier-domaincompressed OTS quantitative phase imaging flow cytometry.It is capable of acquiring intensity and quantitative phase images of cells simultaneously from the compressed data.To evaluate the performance of our method,static microparticles and a corn root cross section are experimentally measured under various compression ratios.Furthermore,to show how our method can be applied in practice,we utilize it in the drug response analysis of breast cancer cells.Experimental results show that our method can acquire high-quality intensity and quantitative phase images of flowing cells at a flowing speed of 1 m/s and a compression ratio of 30%.Combined with machine-learning-based image analysis,it can distinguish drug-treated and drug-untreated cells with an accuracy of over 95%.We believe our method can facilitate cell analysis in both scientific research and clinical settings where both high-throughput and high-content cell analysis is required.

关 键 词：flow STRETCH PHASE 

分 类 号：Q2-33[生物学—细胞生物学] TP391.41[自动化与计算机技术—计算机应用技术]