经阴道超声弹性成像联合血清miR-144-3p、CMTM6在宫颈癌前病变和宫颈癌中的鉴别诊断价值  

Differential diagnostic value of transvaginal ultrasound elastography combined with serum miR-144-3p, CMTM6 for cervical precancerous lesions and cervical cancer

作　　者：王燕 Wang Yan(Department of Ultrasound,Shanxi Children's Hospital,Shanxi Women and Children Hospital,Taiyuan 030001,China)

机构地区：[1]山西省儿童医院,山西省妇幼保健院超声科,太原030001

出　　处：《国际肿瘤学杂志》2024年第8期504-509,共6页Journal of International Oncology

摘　　要：目的探究经阴道超声弹性成像联合血清微RNA-144-3p(miR-144-3p)、趋化素样因子MARVEL跨膜结构域蛋白6(CMTM6)在宫颈癌前病变和宫颈癌中的鉴别诊断价值。方法收集2021年9月至2023年9月山西省妇幼保健院收治的110例宫颈癌患者作为宫颈癌组, 同时选择本院收治的105例宫颈上皮内瘤变(CIN)患者作为CIN组, 另选取同期在本院健康体检者100例作为对照组。采用彩色多普勒超声诊断仪对所有研究对象进行经阴道超声弹性成像检查;实时荧光定量PCR法测定血清miR-144-3p水平;酶联免疫法检测血清CMTM6水平;采用受试者操作特征(ROC)曲线分析超声弹性成像评分联合血清miR-144-3p、CMTM6水平对宫颈癌前病变和宫颈癌的鉴别诊断价值。结果对照组、CIN组和宫颈癌组超声弹性成像评分分别为(1.45±0.22)、(3.16±0.34)、(3.62±0.41)分, 差异有统计学意义(F=1 197.26, P<0.001);miR-144-3p水平分别为1.02±0.12、0.72±0.13、0.57±0.12, 差异有统计学意义(F=357.65, P<0.001);CMTM6水平分别为(15.89±3.46)、(25.82±6.59)、(34.73±8.52)pg/ml, 差异有统计学意义(F=213.05, P<0.001);进一步两两比较发现, 对照组、CIN组和宫颈癌组超声弹性成像评分及CMTM6水平依次升高, miR-144-3p水平依次降低(均P<0.05)。CINⅠ级(n=21)、CINⅡ级(n=44)、CINⅢ级(n=40)患者的超声弹性成像评分分别为(2.86±0.30)、(3.16±0.33)、(3.32±0.35)分, 差异有统计学意义(F=13.20, P<0.001);miR-144-3p水平分别为0.80±0.09、0.73±0.08、0.67±0.07, 差异有统计学意义(F=19.37, P<0.001);CMTM6水平分别为(20.35±2.58)、(25.76±2.71)、(28.76±2.96)pg/ml, 差异有统计学意义(F=62.84, P<0.001);进一步两两比较发现, 随着CIN分级(Ⅰ级、Ⅱ级和Ⅲ级)的升高, CIN患者超声弹性成像评分及CMTM6水平依次升高, miR-144-3p水平依次降低(均P<0.05)。国际妇产科联盟(FIGO)分期为Ⅰ期(n=36)、Ⅱ期(n=42)、Ⅲ~Ⅳ期(n=32)患者的超声弹性成像评分分别Objective:To explore the differential diagnostic value of transvaginal ultrasound elastography combined with serum microRNA-144-3p(miR-144-3p)and CKLF-like MARVEL transmembrane domain containing protein 6(CMTM6)for cervical precancerous lesions and cervical cancer.Methods:A total of 110 patients with cervical cancer admitted to Shanxi Women and Children Hospital from September 2021 to September 2023 were collected as cervical cancer group,105 patients with cervical intraepithelial neoplasia(CIN)admitted to our hospital were selected as the CIN group,another 100 healthy individuals who underwent physical examinations in hospital during the same period were regarded as the control group.Color Doppler ultrasound diagnostic instrument was applied to all research objects for transvaginal ultrasound elastography examination,real-time fluorescence quantitative PCR was applied to measure serum miR-144-3p level,enzyme-linked immunosorbent assay was applied to detect serum CMTM6 level,and receiver operator characteristic(ROC)curve was applied to analyze the significance of differential diagnosis of ultrasound elastography score combined with serum miR-144-3p and CMTM6 levels for cervical precancerous lesions and cervical cancer.Results:The ultrasound elastography scores of control group,CIN group and cervical cancer group were 1.45±0.22,3.16±0.34 and 3.62±0.41,respectively,with a statistically significant difference(F=1197.26,P<0.001).The levels of miR-144-3p were 1.02±0.12,0.72±0.13,0.57±0.12,respectively,with a statistically significant difference(F=357.65,P<0.001).The CMTM6 levels were(15.89±3.46),(25.82±6.59)and(34.73±8.52)pg/ml,respectively,with a statistically significant difference(F=213.05,P<0.001).Further pairwise comparison showed that the elastography scores and CMTM6 levels in the control group,CIN group and cervical cancer group were increased successively,while the levels of miR-144-3p were decreased successively(all P<0.05).The ultrasound elastography scores of CINⅠgrade(n=21),CINⅡgrade(n=44)and

关 键 词：宫颈肿瘤 宫颈癌前病变 超声弹性成像 微RNAS 趋化素样因子MARVEL跨膜结构域蛋白6 

分 类 号：R445.1[医药卫生—影像医学与核医学] R737.33[医药卫生—诊断学] R446.11[医药卫生—临床医学]