基于生物信息学方法设计鼠伤寒沙门氏菌OmpC-FliC多表位疫苗  

Design of Salmonella typhimurium OmpC-FliC multi-epitope vaccine based on bioinformatics method

作　　者：刘意 张喜悦[1,2] 李昕 向祝 郎雨辰 宋时萍 赵格 曲志娜[1,2] 刘立恒 王君玮[1,2] LIU Yi;ZHANG Xiyue;LI Xin;XIANG Zhu;LANG Yuchen;SONG Shiping;ZHAO Ge;QU Zhina;LIU Liheng;WANG Junwei(Pathogenic Microorganism Monitoring Unit,China Animal Health and Epidemiology Center,Qingdao 266032,China;Risk Assessment Laboratory(Qingdao)of Livestock and Poultry Product Quality and Safety,Ministry of Agriculture and Rural Affairs,Qingdao 266032,China;College of Animal Science and Technology,Jiangxi Agricultural University,Nanchang 330045,China)

机构地区：[1]中国动物卫生与流行病学中心致病微生物监测室,山东青岛266032 [2]农业农村部畜禽产品质量安全风险评估实验室(青岛),山东青岛266032 [3]江西农业大学动物科学技术学院,南昌330045

出　　处：《黑龙江畜牧兽医》2024年第18期104-109,116,135,136,共9页Heilongjiang Animal Science And veterinary Medicine

基　　金：“十四五”国家重点研发计划项目“牛奶安全主要风险因子评估与预警防控关键技术”(2022YFD1301003)。

摘　　要：为了设计鼠伤寒沙门氏菌OmpC-FliC多表位疫苗,试验在NCBI网站的Protein数据库中获取OmpC和FliC蛋白的氨基酸序列,采用Expasy服务器分析其理化性质,通过在线工具AllerTopV.2.0和Vexijen分析其抗原性和致敏性,应用NPS~@服务器分析二级结构,应用IEDB在线工具分析OmpC和FliC的B、T细胞表位。选取优势表位设计鼠伤寒沙门氏菌OmpC-FliC多表位疫苗,对其理化性质、抗原性、致敏性及三级结构进行预测。结果表明:OmpC和FliC均是亲水性蛋白,无致敏性,抗原性较高;OmpC和FliC蛋白均筛选出3个B细胞优势表位,2个细胞毒性T细胞(CTL细胞)优势表位,1个辅助T细胞(Th细胞)优势表位。设计的多表位疫苗为亲水性的稳定蛋白,抗原性预测的分数为1.1422分,且为非致敏原,预测三级结构模型的可信度大。说明鼠伤寒沙门氏菌OmpC-FliC多表位疫苗对动物无致敏性,且具有良好的抗原性。In order to design a Salmonella typhimurium OmpC-FliC multi-epitope vaccine,in the experiment,the amino acid sequences of OmpC and FliC proteins were obtained from the Protein database on the NCBI website;the physicochemical properties were analyzed using the Expasy server;the antigenicity and sensitization were analyzed by the online tool AllerTopV.2.0 and Vexijen;the secondary structure was analyzed by applying the NPS~@server;and the IEDB online tool was applied to analyze and B and T cell epitopes of OmpC and FliC.The dominant epitopes were selected to design Salmonella typhimurium OmpC-FliC multi-epitope vaccine,and their physicochemical properties,antigenicity,sensitization and tertiary structure were predicted.The results showed that both OmpC and FliC were nonallergenic hydrophilic proteins with high antigenicity;both OmpC and FliC proteins were screened for three B-cell-dominant epitopes,two cytotoxic T-cell(CTL cell)dominant epitopes and one helper T-cell(Th cell)dominant epitope.The designed multi-epitope vaccine was a hydrophilic,stable protein,with an antigenicity prediction score of 1.1422,and was a non-sensitizer;the predicted tertiary structural model had high confidence.The results suggested that the Salmonella typhimurium OmpC-FliC multi-epitope vaccine was non-allergenic and had good antigenicity in animals.

关 键 词：沙门氏菌 生物信息学 OMPC FLIC 表位 疫苗 

分 类 号：S852.5[农业科学—基础兽医学] Q78[农业科学—兽医学]