11个柠檬砧木种质对淹水胁迫的生理响应及耐涝性评价  

Physiological responses of eleven lemon rootstock germplasms to waterlogging stress and their waterlogging tolerance evaluation

作　　者：余智城[1] 林秀香[1] 林秋金[1] 谢晓清[1] 陈振东[1] YU Zhicheng;LIN Xiuxiang;LIN Qiujin;XIE Xiaoqing;CHEN Zhendong(Fujian Institute of Tropical Crops,Zhangzhou,Fujian,363000,China)

机构地区：[1]福建省热带作物科学研究所,福建漳州363001

出　　处：《中国南方果树》2024年第5期18-23,共6页South China Fruits

基　　金：漳州市自然科学基金项目(ZZ2020J15)资助。

摘　　要：探究不同砧木种质植株对淹水胁迫的生理响应及耐涝性,可为柠檬耐涝砧木选育及抗涝机理研究提供理论依据。以11个柠檬砧木种质当年生实生苗为供试材料,设置正常水分管理(对照)和持续淹水处理,通过测定相对电导率、叶绿素、可溶性蛋白、可溶性糖、丙二醛含量和过氧化氢酶活性等指标研究各种质对淹水胁迫的生理响应,进一步通过主成分分析和隶属函数法对各种质的耐涝性进行综合评价,最后根据综合评价值对砧木种质进行聚类分析。结果表明,淹水胁迫后,不同砧木种质的相对电导率均增加,叶绿素和可溶性蛋白含量均降低,可溶性糖、丙二醛含量和过氧化氢活性在不同种质中呈现升高或降低的变化趋势。在供试种质中,白[木黎]檬1号、酸橙和香橙强耐涝,泰国香橼和白[木黎]檬2号较强耐涝,大果红[木黎]檬、酸柚、酸桔和小青柠中等耐涝,红柠檬和粗柠檬不耐涝。Exploring the physiological response and tolerance of different rootstock germplasm plants to waterlogging stress could provide theoretical basis for the breeding of waterlogging tolerant lemon rootstock and the study of waterlogging resistance mechanisms.One-year-old seedlings of eleven rootstocks were used as experimental material,normal water management(control)and consistent flooding treatment were set up.The physiological response of the tested germplasms to waterlogging stress was studied by measuring relative conductivity,the content of chlorophyll,soluble protein,soluble sugar,and malondialdehyde,and catalase activity.The waterlogging tolerance of each material was further evaluated by principal component analysis and membership function method.Finally,the rootstocks were clustered according to the comprehensive evaluation value.The results showed that after waterlogging stress treatment,the relative conductivity of different rootstocks increased,the contents of chlorophyll and soluble protein decreased,and the contents of soluble sugar,malondialdehyde and hydrogen peroxide activity increased or decreased in different materials.Among the tested germplasms,the waterlogging tolerance of Bailimeng No.1,Suancheng and Xiangcheng was very strong,and that of Taiguoxiangyuan and Bailimeng 2 was stronger,followed by Daguohonglimeng,Suanyou,and Xiaoqingning with moderate waterlogging tolerance.Hongningmeng and Cuningmeng were not tolerant to waterlogging.

关 键 词：柠檬 砧木 耐涝性 主成分分析 隶属函数法 综合评价 

分 类 号：S666.5[农业科学—果树学]