龙珠果NBS-LRR类抗病基因同源序列的克隆与分析  

Cloning and analysis of homologous sequences of NBS-LRR disease resistant genes of Passiflora foetida L.

作　　者：杨翠凤 滕峥 廖志东 韦春 YANG Cuifeng;TENG Zheng;LIAO Zhidong;WEI Chun(Agriculture and Food Engineering College,Baise University/Guangxi Key Laboratory of Biology for Mango/Industrial College of Subtropical Characteristic Agriculture,Baise,Guangxi,533000,China)

机构地区：[1]百色学院农业与食品工程学院/广西芒果生物学重点实验室/亚热带特色农业产业学院,广西百色533000

出　　处：《中国南方果树》2024年第5期63-69,73,共8页South China Fruits

基　　金：广西自然科学基金(2022GXNSFBA035462、2023GXNSFBA026046);广西科技基地和人才专项(2020AC20047);广西一流学科建设项目(农业资源与环境,桂教科研[2022]1号)资助。

摘　　要：龙珠果Passiflora foetida L.为西番莲属的近缘野生种,具有抗逆性强、适应性广等特点,是西番莲抗病育种潜在的抗病基因资源库。根据已知植物抗病基因NBS-LRR保守结构域设计简并引物,以龙珠果为试材,克隆龙珠果基因组中NBS-LRR类抗病基因同源序列(RGAs),并进行聚类分析。结果表明,获得的龙珠果NBS-LRR类抗病基因序列有TIR和non-TIR两种类型,其中TIR-NBS-LRR类型5条,编号为LRGA-1~LRGA-5;non-TIR-NBS-LRR类型7条,编号为LRGA-6~LRGA-12。聚类分析发现,LRGA-1~LRGA-5与已知的烟草抗TMV(烟草花叶病毒)基因N及亚麻抗锈病基因L6同源性较高(相似性59%~86%),LRGA-6~LRGA-12与已知拟南芥抗丁香假单胞病菌基因RPM1及水稻抗白叶枯病基因Xa-1同源性较高(相似性51%~70%),尤其是LRGA-3与LRGA-5序列,与多种植物抗TMV蛋白基因编码的氨基酸相似性极高,推测可能为西番莲属抗TMV基因。Passiflora foetida L.is a closely related wild species of the cultivated species of genus Passiflora,with strong stress resistance and wide adaptability.It is a potential gene resource pool for disease resistance breeding of Passiflora.Degenerate primers were designed based on the conserved domain of the known plant disease resistance gene NBS-LRR to clone NBS-LRR class disease resistance gene homologous sequences(RGAs)from the P.foetida L.genome using P.foetida L.as the test material,and perform cluster analysis.The results showed that there were two types of P.foetida L.NBS-LRR disease resistance gene sequences,TIR and non TIR.Among them,there were 5 TIR-NBS-LRR types of sequences,numbered from LRGA-1 to LRGA-5,and 7 non TIR-NBS-LRR types of sequences,numbered from LRGA-6 to LRGA-12.Cluster analysis revealed that LRGA-1,LRGA-2,LRGA-3,LRGA-4 and LRGA-5 had high homology(59%-86%)with the known tobacco resistance gene N and flax rust resistance gene L6,while LRGA-6,LRGA-7,LRGA-8,LRGA-9,LRGA-10,LRGA-11 and LRGA-12 had high homology(51%-70%)with the known Arabidopsis resistance gene RPM1 and rice resistance gene Xa-1.In particular,LRGA-3 and LRGA-5 sequences had extremely high similarity of amino acid sequences with various plant resistance genes to tobacco mosaic virus(TMV),suggesting that they may be anti TMV genes in Passiflora.

关 键 词：龙珠果 西番莲 NBS-LRR 抗病基因 同源性 

分 类 号：S667.9[农业科学—果树学]