罗汉果组培块茎诱导及离体再生  

Induction and in vitro regeneration of Siraitia grosvenorii tubers

作　　者：曾成 莫海萍[2] 庞秋凌 娄兵海 邓光宙 张松 李伯林[2] 苏玉卿 ZENG Cheng;MO Haiping;PANG Qiuling;LOU Binghai;DENG Guangzhou;ZHANG Song;LI Bolin;SU Yuqing(Guangxi Key Laboratory of Germplasm Innovation and Utilization of Specialty Commercial Crops in North Guangxi/Guangxi Academy of Specialty Crops,Guilin,Guangxi,541004,China;College of Life Science,Guangxi Normal University,Guilin,Guangxi,541004,China)

机构地区：[1]广西桂北特色经济作物种质创新与利用重点实验室/广西特色作物研究院,广西桂林541004 [2]广西师范大学生命科学学院,广西桂林541004

出　　处：《中国南方果树》2024年第5期95-100,共6页South China Fruits

基　　金：广西特色作物试验站项目(桂TS2022007);广西农业科技自筹经费项目(Z2022125、Z2022126、Z2024043);广西特色作物研究院科研基金(2023B003);广西自然科学基金(2024GXNSFBA999268)资助。

摘　　要：为满足罗汉果种质资源保存和优良种苗生产的需求,以“伯林二号”罗汉果带芽茎段为外植体,采用添加不同浓度的萘乙酸(NAA)、6-苄基腺嘌呤(6-BA)和多效唑的MS培养基,诱导块茎、离体保存和恢复生长,并移栽种植,比较不同处理的块茎诱导率、存活率、株高、叶片数等。结果表明,供试罗汉果块茎诱导和离体保存的最佳培养基为1/2MS+0.05 mg/L NAA+0.50 mg/L 6-BA+1.00 mg/L多效唑,块茎诱导率为97.67%,540 d离体保存存活率为87.00%;诱导块茎恢复生长最佳培养基为MS+0.5 mg/L NAA+1.00 mg/L 6-BA,存活率99.00%,根和不定芽长势良好。移栽试验发现,诱导块茎移栽存活率达到97.33%,高于组培苗,且植株长势最佳,诱导块茎恢复生长后茎段培养的组培苗和常规方法培养的组培苗移栽表现无明显差异,长势良好。In order to meet the needs of germplasm conservation as well as the production of excellent seedlings of Siraitia grosvenorii,stem segment with buds of Bolin No.2 S.grosvenorii was used as explant,and MS medium with different concentrations of naphthalene acetic acid(NAA),6-benzyl adenine(6-BA)and paclobutrazol was used to induce tuber and for in vitro preservation and growth recovery,and then transplanting.The tuber induction rate,survival rate,plant height and number of leaves under different treatments were compared.The results showed that the optimal medium for tuber induction and in vitro preservation of the test sample of S.grosvenorii was 1/2 MS+0.05 mg/L NAA+0.50 mg/L 6-BA+1.00 mg/L paclobutrazol,with the tuber induction rate of 97.67%,and survival rate of 87.00% after 540 days of in vitro preservation.The optimal medium for induced tuber growth recovery was MS+0.5 mg/L NAA+1.00 mg/L 6-BA,with the survival rate of 99.00%,showing good growth of roots and adventitious buds.The transplanting experiment revealed that the survival rate of the induced tubers reached 97.33%,higher than that of tissue culture seedlings,with the best plant growth.There was no significant difference in the transplanting performance between the tissue culture seedlings of stem segment culture from growth recovered inducing tuber and conventional tissue culture seedlings,both of them exhibited good growth.

关 键 词：罗汉果 诱导块茎 离体再生 组织培养 存活率 

分 类 号：S668.9[农业科学—果树学]