黑漆实蝇线粒体基因组基因结构分析  

Mitochondrial Gene Structure Analysis of Bactrocera (Zeugodacus) scutellaris

作　　者：黄振 郭琼霞 HUANG Zhen;GUO Qiong-xia(Changle Airport Customs of Fuzhou,Fuzhou,Fujian 350209;Fuzhou Customs Technology Center,Fuzhou,Fujian 350001)

机构地区：[1]福州长乐机场海关,福建福州350209 [2]福州海关技术中心,福建福州350001

出　　处：《安徽农业科学》2024年第19期77-80,共4页Journal of Anhui Agricultural Sciences

基　　金：福建省自然科学基金项目(2011J01066,2012JO1061);福州海关科研项目(FK2022-09)。

摘　　要：为探究黑漆实蝇线粒体基因组基因结构,基于测定的黑漆实蝇的线粒体全基因组,采用DNAMAN V6软件测算线粒体全基因组13个蛋白编码基因(PCGs),22个trnA基因AT skew=(A-T)/(A+T)、GC skew=(G-C)/(G+C)的值,分析黑漆实蝇核苷酸的偏移率;使用Win32 CodonW软件分析蛋白质基因密码子的使用个数以及同义相对密码子RSCU值的使用频率;利用MITOS WebServer和tRNAscan-SE 2.0软件对trnA的二级结构进行分析,为物种诊断、系统发育和进化生物学提供依据。In order to explore the mitochondrial genome gene structure of Bactrocera(Zeugodacus)scutellaris,based on the measured mitochondrial whole genome of B.(Z.)scutellaris,the values of ATskew=(A-T)/(A+T)and GC skew=(G-C)/(G+C)of 13 protein-coding genes(PCGs)and 22 trn A genes in the whole mitochondrial genome were measured by DNAMAN V6 software.Win32 CodonW software was used to analyze the number of protein gene codons and the frequency of synonymous relative codon RSCU values.MITOS WebServer and trn Ascan-SE 2.0 software were used to analyze the secondary structure of trn A,providing evidence for species diagnosis,phylogeny and evolutionary biology.

关 键 词：黑漆实蝇 线粒体全基因组 相对密码子使用频率 trnA基因二级结构 

分 类 号：S433[农业科学—农业昆虫与害虫防治]