辣椒贮藏性病害病原菌的分离鉴定及拮抗菌对其抑制作用研究  

Isolation and Identification of Pathogenic Bacteria of Storage Venereal Diseases in Capsicum and Study on Their Inhibition by Antagonistic Bacteria

作　　者：胡秀虹 张廷辉 欧明云 宋敏 HU Xiu-hong;ZHANG Ting-hui;OU Ming-yun(School of Life and Health Science,Kaili University,Kaili,Guizhou 556011;Qiandongnan Food and Drug Inspection and Testing Center,Kaili,Guizhou 556011)

机构地区：[1]凯里学院大健康学院,贵州凯里556011 [2]黔东南州食品药品检验检测中心,贵州凯里556011

出　　处：《安徽农业科学》2024年第19期168-171,共4页Journal of Anhui Agricultural Sciences

基　　金：2022年国家级大学生创新创业训练计划项目(20221066-9044);2022年省级“金课”(一流课程)建设项目(SJJK202221);2022年度黔东南州基础研究计划项目(黔东南科合基础[2022]05号);凯里学院2023年校级专项课题(2023XJZX0304)。

摘　　要：以自然病变的羊角辣椒为原料,采用组织培养法及回接法从中分离筛选优势病原菌。通过形态学观察和ITS全序列分析对优势病原菌进行鉴定,并研究了一株拮抗细菌Bacillus subtilis对优势病原菌的抑制作用。结果表明:导致辣椒在贮藏过程中发生病变的优势病原菌为1株真菌,经鉴定为Botrytis cinerea。研究发现,生防菌Bacillus subtilis对病原菌Botrytis cinerea具有良好的抑制效果,抑菌率可达87.8%。The dominant pathogenic bacteria were isolated and screened by tissue culture method and tieback method from natural pathological changes of capsicum chinensis.The dominant pathogenic bacteria were identified by morphological observation and ITS sequence analysis.The inhibitory effect of an antagonistic bacterium—Bacillus subtilis on the dominant pathogenic bacteria was studied.The results showed that the dominant pathogenic bacteria that caused the pathological changes of capsicum during storage was a fungus strain,which was identified as Botrytis cinerea.It was found that Bacillus subtilis had good inhibitory effect on Botrytis cinerea with inhibition rate of 87.8%.

关 键 词：辣椒 病原菌 分离鉴定 生防菌 拮抗作用 

分 类 号：Q934.1[生物学—微生物学]