氨氮胁迫对厌氧处理高浓度有机氮废水的影响  

The effect of ammonia nitrogen stress on anaerobic digestion treatment of high concentration organic nitrogen wastewater

作　　者：党澎泽 谈新伟 李祥[1,2,3] 袁砚 黄勇[1,2] 李鹏飞[4] 冯震 DANG Peng-ze;TAN Xin-wei;LI Xiang;YUAN Yan;HUANG Yong;LI Peng-fei;FENG Zheng(School of Environmental Science and Engineering,Suzhou University of Science and Technology,Suzhou 215009,China;Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment,Suzhou University of Science and Technology,Suzhou 215009,China;Suzhou Tianjun Environmental Technology Co.,Ltd.,Suzhou 215100,China;Suzhou Keruide Energy Conservation Environmental Technology Co.,Ltd.,Suzhou 215011,China)

机构地区：[1]苏州科技大学环境科学与工程学院,江苏苏州215009 [2]苏州科技大学江苏水处理技术与材料协同创新中心,江苏苏州215009 [3]苏州天竣环境科技有限公司,江苏苏州215100 [4]苏州科锐德节能环境科技有限公司,江苏苏州215011

出　　处：《中国环境科学》2024年第10期5453-5460,共8页China Environmental Science

基　　金：国家自然科学基金资助项目(51938010);苏州市科技计划项目-民生科技项目(SS202025);江苏省水处理技术与材料协同创新中心(XTCXSZ2022-1);江苏省环境科学与工程重点实验室(JSHJZDSYS-202004);江苏省研究生科研与实践创新计划项目(KYCX23_3345)。

摘　　要：以实际制膜废水为例,探究了氨胁迫下高浓度有机氮废水甲烷化及微生物群落的动态响应,明确了氨抑制阈值,抑制形态,抑制机制和应对策略.结果表明,氨对AD处理高浓度有机氮废水的主要氨抑制形态为游离氨(FA),抑制阈值为(145±10)mg/L,半抑制浓度为(244±10)mg/L.在AD处理高浓度有机氮废水时,乙酸营养型产甲烷菌Methanosaeta,甲基营养型产甲烷菌Methanomassiliicoccus和Methanomethylovorans是主要产甲烷微生物.Methanosaeta和Methanomethylovorans分别在氨氮高于300mg/L和900mg/L时活性下降,同时产氢产二氧化碳相关功能微生物在FA高于140mg/L时受到抑制,使含碳数大于1的小分子有机物在系统内累积,最终导致高FA条件下化学需氧量去除率降低和氨氮释放不完全.在受到高氨抑制后,可通过调控pH值的方式实现系统的恢复,但无法通过微生物驯化的方式缓解.Using actual membrane wastewater as an example,this study explored the dynamic responses of methanogenesis and microbial communities in high-concentration organic nitrogen wastewater under ammonia stress,clarifying the ammonia inhibition threshold,inhibition form,inhibition mechanisms,and response strategies.The results indicate that the primary form of ammonia inhibition in anaerobic digestion(AD)treatment of high-concentration organic nitrogen wastewater is free ammonia(FA),with an inhibition threshold of(145±10)mg/L and a half-maximal inhibitory concentration of(244±10)mg/L.During AD treatment of high-concentration organic nitrogen wastewater,the key methanogenic microorganisms include Methanosaeta(acetotrophic methanogens),Methanomassiliicoccus and Methanomethylovorans(methylotrophic methanogens).The activities of Methanosaeta and Methanomethylovorans decline at ammonia nitrogen levels above 300mg/L and 900mg/L,respectively.Simultaneously,the functional microbes related to hydrogen and carbon dioxide production are inhibited when FA exceeds 140mg/L.This leads to the accumulation of small organic molecules with more than one carbon atom in the system,ultimately resulting in reduced chemical oxygen demand removal efficiency and incomplete release of ammonia nitrogen under high free ammonia(FA)conditions.After severe ammonia inhibition,recovery of the system can be achieved by adjusting the pH,but not through microbial acclimatization.

关 键 词：N N-二甲基甲酰胺 厌氧甲烷发酵 半抑制浓度 游离氨 抑制阈值 控制策略 

分 类 号：X703.5[环境科学与工程—环境工程]