H10N3禽流感病毒安徽分离株遗传多样性研究  

Genetic Diversity of H10N3 Avian Influenza Virus Isolated from Anhui Province,China

作　　者：李珊 董卉 姚雪 张春鸽 邢薇佳 李娟 史卫峰 LI Shan;DONG Hui;YAO Xue;ZHANG Chunge;XING Weijia;LI Juan;SHI Weifeng(Key Laboratory of Emerging Infectious Diseases in Universities of Shandong,Shandong First Medical University&Shandong Academy of Medical Sciences,Jinan 25117,China;Department of Pathogen Biology,School of Clinical and Basic Medical Sciences,Shandong First Medical University&Shandong Academy of Medical Sciences,Jinan 250l17,China;School of Public Health,Shandong First Medical University&Shandong Academy of Medical Sciences,Jinan 250117,China;CAS Key Laboratory of Pathogen Microbiology and Immunology,Institute of Microbiology,Center for Influenza Research and Early-warning(CASCIRE),CAS-TWAS Center of Excellence for Emerging Infectious Diseases(CEEID),Chinese Academy of Sciences,Beijing 100101,China)

机构地区：[1]山东第一医科大学(山东省医学科学院)山东省高等学校新发传染病重点实验室,济南250117 [2]山东第一医科大学(山东省医学科学院)临床与基础医学院(基础医学研究所)病原生物学系,济南250117 [3]山东第一医科大学(山东省医学科学院)公共卫生与健康管理学院,济南250117 [4]中国科学院流感研究与预警中心,中国科学院微生物研究所,病原微生物与免疫学重点实验室,北京100101

出　　处：《病毒学报》2024年第5期1022-1032,共11页Chinese Journal of Virology

基　　金：泰山学者青年专家计划项目(项目号:tsqn202211217);山东第一医科大学“学术提升计划”项目(项目号:2019QL006)。

摘　　要：2021年4月我国江苏确诊全球第一例人感染H10N3禽流感病例,研究我国活禽市场H10N3亚型禽流感病毒(Avian influenza virus,AIV)的遗传多样性和分子特征,将为理解人感染H10N3病毒的进化来源、评估其溢出风险提供理论依据。为此,2021年6-12月我们对安徽地区活禽市场销售家禽及环境中H10N3 AIV进行实时荧光定量RT-PCR检测,共分离鉴定到6株H10N3病毒(AH01-AH06),其中1株为11月份环境样本分离株,其余5株为6月、11月和12月鸡咽拭子分离株。高通量测序和生物信息学分析表明,同一活禽市场分离到的毒株表现出一定的遗传多样性和重配模式差异:4株H10N3分离株(AH01-AH04)间核苷酸同源性均高于99.00%,而A/chicken/Anhui/11-30-YHZGS024-O/2021(AH05)株的MP基因片段和A/chicken/Anhui/06-26-YHZGS006-O/2021(AH06)株的PA、MP基因片段与其他分离株的核苷酸同源性较低,均低于97.04%。系统发育分析显示,本研究6株H10N3分离株与人源H10N3病毒的HA和NA基因均位于欧亚进化分支,它们的内部基因都来自H9N2亚型AIV。特别是分离株AH06与人感染H10N3病毒八个基因均位于同一进化分支,而分离株AH01-AH05的PA基因与人感染H3N8和H9N2毒株、MP基因与人感染H9N2毒株的亲缘关系较近。分子遗传特征分析显示,6株H10N3 AIV的血凝素蛋白均携带G228S等哺乳动物适应性突变,提高了对人源受体的结合能力。综上,本研究证实了禽源H10N3病毒在安徽省活禽市场的持续流行,揭示了禽源H10N3病毒的遗传多样性以及新型感染人的H10N3在我国家禽中传播,强调其携带多个哺乳动物适应性突变,为评估H10N3 AIV潜在公共卫生风险提供了理论依据。In April 2021,the first human case of infection by the H10N3 subtype avian influenza virus(AIV)was identified in Jiangsu,China.The genetic diversity and molecular characterizations of H10N3 subtype AIV from live-poultry markets in China were studied.This strategy could enable understanding of the evolutionary origin of human-infecting H10N3 AIV and assessment of its spillover risk from poultry to humans.From June to December 2021,real-time reverse transcription-quantitative polymerase chain reactions were undertaken to detect H10N3 subtype AIV in poultry markets and environments in Anhui,China.Six strains(AH01–AH06)of H10N3 subtype AIV were isolated and identified.The whole-genome sequences of these viruses were amplified by high-throughput sequencing,and their genetic characterizations were analyzed.One of the six H10N3 isolates was found in an environmental sample collected in November 2021,and the remaining five were from chicken-throat swabs collected in June,November,and December 2021.Strains isolated from the same live-poultry market exhibited genetic diversity and differences in reassortment patterns.Among the six H10N3isolates,the eight gene segments of four isolates(AH01–AH04)had nucleotide homology>99.00%.The PA gene segment of A/chicken/Anhui/11-30-YHZGS024-O/2021(AH05),and the PA and MP gene segments of A/chicken/Anhui/06-26-YHZGS006-O/2021(AH06)shared low homology with those of the other four H10N3 isolates,with genetic identities<97.04%.Phylogenetic analyses showed that the HA and NA genes of the six H10N3 isolates and human H10N3 strain fell within the Eurasian lineage,and their internal genes were derived from the AIV of the H9N2 subtype.The eight segments of AH06 were placed in the same branch with the human-infecting H10N3 strain,while the PA gene of other isolates was grouped with humaninfecting H3N8 and H9N2 AIVs:the MP gene was more closely related to human-infecting H9N2 AIVs.Molecular characterization showed that all six H10N3 AIV strains had mammalian adaptive mutations such as G228S,w

关 键 词：禽流感病毒 活禽市场 H10N3 遗传多样性 

分 类 号：R373.1[医药卫生—病原生物学]