HRM技术快速检测新冠病毒变异株  

Rapid Detection of SARS-CoV-2 Variants by HRM Technology

作　　者：周玉真 程悦 谢汶君 何迅 沈丹芸 陈恒 ZHOU Yuzhen;CHENG Yue;XIE Wenjun;HE Xun;SHENG Danyun;CHEN Heng(Chengdu Workstation for Emerging Infectious Disease Control and Prevention,Chinese Academy of Medical Sciences,Chengdu 610000 China;Chengdu Center for Disease Control and Prevention,Chengdu 610o00,China)

机构地区：[1]中国医学科学院成都新发突发传染病防治工作站,成都610000 [2]成都市疾病预防控制中心,成都610000

出　　处：《病毒学报》2024年第5期1076-1081,共6页Chinese Journal of Virology

基　　金：四川省医学青年创新科研课题计划(项目号:Q22017),题目:新型冠状病毒宿主内适应性进化及其应用研究;2022年成都市医学科研课题(项目号:2022056),题目:基于突变位点库的新冠病毒变异株快速检测及溯源平台。

摘　　要：建立基于高分辨率融解曲线(High resolution melting,HRM)的快速检测新冠病毒变异株的方法,为新冠变异株快速检测提供技术支撑。针对新冠病毒S基因的氨基酸突变位点G339H/D、D796Y、V83A设计HRM引物,将新冠病毒实时定量荧光PCR(RT-PCR)阳性的核酸逆转录后进行HRM分析,并对33份新冠病毒RT-PCR阳性核酸标本进行检测,与二代测序结果进行比较。建立的HRM方法能区分Delta、Omicron及XBB亚型,33份标本的HRM分析结果显示其中2份为Delta株,31份为Omicron株,12份为XBB亚型。HRM分析结果与二代测序结果一致。本实验建立的HRM方法能够准确检测出新冠病毒变异株中的Delta、Omicron及XBB亚型,可应用于快速鉴定新冠病毒型别。To:(i)establish a method based on high-resolution melting(HRM)curves for the rapid detection of severe acute respiratory-coronavirus-2(SARS-CoV-2)variants;(ii)provide technical support for the rapid detection of SARS-CoV-2 variants.HRM primers were designed for the amino-acid mutation sites G339H/D,D796Y,and V83A of the S gene of SARS-CoV-2.Detection of the nucleic acids of SARS-CoV-2 was undertaken with real-time reverse transcription-quantitative polymerase chain reaction.Thirty-three samples of SARS-CoV-2-positive nucleic acids were detected and compared with the results of next-generation sequencing.The HRM method could be used to distinguish the Delta,Omicron,and XBB variants of SARS-CoV-2.HRM analyses of 33 samples showed that there were two Delta,31 Omicron,and 12 XBB subtypes of SARS-CoV-2.The results of HRM analyses were consistent with those of next-generation sequencing.Our HRM method could be used to accurately detect the Delta,Omicron,and XBB variants of SARS-CoV-2.

关 键 词：新冠变异株 高分辨率融解曲线 德塔尔株 奥密克戎株 XBB 

分 类 号：R331[医药卫生—人体生理学] R373.1[医药卫生—基础医学]