果王素通过下调结缔组织生长因子表达防治大鼠放射性肺损伤  

Kiwi fruit essence prevents radiation-induced lung injury in rats by down-regulating CTGF expression

作　　者：刘理静 尹勤 贺立洋 魏文婕 周美玲 贺兼斌 LIU Lijing;YIN Qin;HE Liyang;WEI Wenjie;ZHOU Meiling;HE Jianbin(Medical School,Changsha Vocational and Technical College of Civil Affairs,Changsha 410004,China;Department of Geriatric Respiratory,General Hospital of Hunan Medical University,Huaihua 418000,Hunan,China;Huaihua Clinical College,University of South China,Huaihua 418000,Hunan,China)

机构地区：[1]长沙民政职业技术学院医学院,湖南长沙410004 [2]湖南医药学院总医院老年呼吸科,湖南怀化418000 [3]南华大学怀化临床学院,湖南怀化418000

出　　处：《西部医学》2024年第10期1436-1443,共8页Medical Journal of West China

基　　金：湖南省自然科学基金资助项目(2018JJ6045);湖南省教育厅资助科研项目(21C1593);怀化市科技计划资助项目(2017G3301)。

摘　　要：目的动态观察果王素对放射性肺损伤(RILI)大鼠肺组织中结缔组织生长因子(CTGF)蛋白及mRNA表达的影响,探讨果王素是否可通过调节CTGF防治RILI。方法将SD大鼠90只按随机数字表法分为空白组对照组、模型组、果王素低、中、高剂量组(果王素剂量分别为60 mg/kg、120 mg/kg、240 mg/kg)。除空白对照组,其它各组大鼠给予全胸部放射构建大鼠RILI动物模型,在放射前7 d,低、中、高剂量果王素组分别按果王素60 mg/kg、120 mg/kg、240 mg/kg加入0.9%氯化钠,配成溶液1 mL,每日灌胃1次,直至处死;空白对照组与模型组同一时间给予1 mL 0.9%氯化钠灌胃,放疗结束后第14、28、56天分别随机处死各组大鼠6只,HE、Masson染色观察肺组织病理改变,采用免疫组化、Western blot、qRT-PCR检测CTGF蛋白质及mRNA的表达。结果相比模型组,低、中、高剂量果王素干预组肺组织肺泡炎和肺纤维化减轻,肺泡炎及纤维化评分降低,CTGF蛋白及CTGF mRNA表达减少(均P<0.05),低、中、高剂量果王素干预组组间比较,剂量越高上述变化越明显(P<0.05)。结论果王素对RILI具有防治作用,且防治效果与果王素浓度正相关,其机制可能与下调CTGF表达有关。Objective To observe the effect of Kiwi fruit essence on the expression of connective tissue growth factor(CTGF)protein and mRNA in the lung tissue of rats with radiation-induced lung injury(RILI),and explore whether Kiwi fruit essence can prevent and treat RILI by regulating CTGF.Methods 90 SD rats were divided into blank control group,model group,low dose(60 mg/kg)Kiwi fruit essence group,middle dose(120 mg/kg)Kiwi fruit essence group and high dose(240 mg/kg)Kiwi fruit essence group,randomly.Except the blank group,the other groups were treated with whole chest radiation to establish the RILI rat model.Seven days before radiation,the low dose group,middle dose group and high dose groups were added with 0.9%sodium chloride according to 60 mg/kg,120 mg/kg and 240mg/kg Kiwi fruit essence,respectively,to form a total 1mL solution,once a day until the rats were killed.The blank group and the model group were given 0.9%sodium chloride 1mL intragastrically at the same time,and 6 rats were randomly killed in each group on the 14th,28th and 56th day after radiotherapy.Immunohistochemistry,Western blot and qRT-PCR were used to detect the expression of CTGF protein and mRNA.Results Compared with the model group,the degree of alveolitis and pulmonary fibrosis,the score of alveolitis and fibrosis,and the expression of CTGF protein and CTGF mRNA in the 60,120 and 240 mg/kg of Kiwi fruit essence group were reduced(P<0.05 or P<0.01).The changes were more obvious with higher dose(P<0.05 or P<0.01).Conclusion Kiwi fruit essence has a protective and therapeutic effect on RILI,and the protective and therapeutic effect is positively related to the concentration of Kiwi fruit essence,and its mechanism may be related to the down-regulation of CTGF expression.

关 键 词：果王素 放射性肺损伤 结缔组织生长因子 肺纤维化 肺泡炎 

分 类 号：R818[医药卫生—放射医学]