菲牛蛭素对ApoE^(-/-)小鼠动脉粥样硬化体内脂质代谢和血管斑块的影响  

Effects of bufrudin on lipid metabolism and vascular plaque in ApoE^(-/-)mice atherosclerosis

作　　者：黄贵庆 李吉星 黄栎莹 韦文兴 林思 赖宗强 黎渊弘[1] HUANG Guiqing;LI Jixing;HUANG Liying;WEI Wenxing;LIN Si;LAI Zongqiang;LI Yuanhong(The Second Affiliated Hospital of Guangxi Medical University,Nanning,Guangxi 530007,China;Nanning Animal Disease Prevention and Control Center,Nanning,Guangxi 530007,China)

机构地区：[1]广西医科大学第二附属医院,广西南宁530007 [2]南宁市动物疫病预防控制中心,广西南宁530007

出　　处：《现代医药卫生》2024年第19期3241-3247,3252,共8页Journal of Modern Medicine & Health

基　　金：广西自然科学基金面上项目(2020GXNSFAA238042)。

摘　　要：目的探讨菲牛蛭素对ApoE^(-/-)小鼠动脉粥样硬化体内脂质代谢和血管斑块的影响。方法将24只高脂饮食饲养的雄性ApoE^(-/-)小鼠随机分为模型组,菲牛蛭素高、低剂量给药组和辛伐他汀阳性给药组,每组6只。另取6只C57BL/6J小鼠作为空白对照组,正常饲料喂养。之后连续给药干预8周,末次给药后,检测血脂水平,HE染色及Masson染色观察主动脉血管病理学改变;免疫组化染色分别观察菲牛蛭素对小鼠主动脉粥样斑块中增殖细胞核抗原(PCNA)、细胞凋亡蛋白-3(caspase-3)蛋白的影响。结果与空白对照组比较,模型组小鼠血清总胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白胆固醇(LDL-C)水平显著升高,高密度脂蛋白胆固醇(HDL-C)水平显著降低,差异均有统计学意义(P<0.01);与模型组比较,菲牛蛭素高、低剂量组小鼠血清TC、TG和LDL-C水平显著降低,HDL-C水平显著升高,差异均有统计学意义(P<0.01)。Masson染色显示,与模型组比较,菲牛蛭素高、低剂量组小鼠血管斑块中的胶原含量显著增加,差异均有统计学意义(P<0.01)。免疫组化染色结果显示,与空白对照组比较,模型组小鼠主动脉粥样斑块PCNA蛋白表达显著下调,caspase-3蛋白表达显著上调,差异均有统计学意义(P<0.01);与模型组比较,菲牛蛭素组小鼠主动脉粥样斑块PCNA蛋白表达显著上调,菲牛蛭素高剂量组的caspase-3蛋白表达显著下调,差异均有统计学意义(P<0.05)。结论菲牛蛭素可以通过降低ApoE^(-/-)小鼠血清血脂水平,延缓平滑肌细胞凋亡,稳定血管斑块等方面来抑制动脉粥样硬化的发展。Objective To investigate the effect of bufrudin on lipid metabolism and vascular plaques in ApoE^(-/-)mice with atherosclerosis.Methods A total of 24 male ApoE^(-/-)mice fed with high-fat diet were randomly divided into the model group,the high-dose and the low-dose groups of bufrudin,and the simvastatin positive group,with six mice in each group.Another six C57BL/6J mice were taken as the blank control group and fed with normal diet.After continuous administration for eight weeks,the blood lipid level was detected after the last administration,and the pathological changes of aortic vessels were observed by HE staining and Masson staining.Immunohistochemical staining was used to observe the effects of bufrudin on proliferating cell nuclear antigen(PCNA)and caspase-3 protein in aortic atherosclerotic plaques of mice.Results Compared with the blank control group,the levels of serum total cholesterol(TC),triglyceride(TG)and low density lipoprotein cholesterol(LDL-C)in the model group were significantly increased,and the level of high density lipoprotein cholesterol(HDL-C)was significantly decreased,and differences were statistically significant(P<0.01).Compared with the model group,the levels of serum TC,TG and LDL-C in the high-dose and low-dose groups of bufrudin were significantly decreased,and the level of HDL-C was significantly increased,the differences were statistically significant(P<0.01).Masson staining showed that compared with the model group,the collagen content in the vascular plaque of mice in the high and low dose groups of bufrudin was significantly increased,and the difference was statistically significant(P<0.01).The results of immunohistochemical staining showed that compared with the blank control group,the expression of PCNA protein in the aortic atherosclerotic plaque of the model group was significantly down-regulated,and the expression of caspase-3 protein was significantly up-regulated,the differences were statistically significant(P<0.01).Compared with the model group,the expression of PCN

关 键 词：动脉粥样硬化 菲牛蛭素 血脂水平 血管斑块 血管平滑肌细胞 

分 类 号：R96[医药卫生—药理学]