无花果内生菌Alternaria sp.QDFB-2菌丝体化学成分的研究  

Study on the chemical constituents in the mycelium of endophyte Alternaria sp.QDFB-2 from Ficus carica L.

作　　者：徐力 刘汉山[2] 叶桐 郝增君 王凤舞[5] 梁詠文 申丽[3,4] XU Li;LIU Hanshan;YE Tong;HAO Zengjun;WANG Fengwu;LIANG Yongwen;SHEN Li(Yangzhou Institute for Food and Drug Control Department,Yangzhou 225001,China;Jiangsu Provincial Corps Hospital of Chinese People's Armed Police Forces,Yangzhou 225003,China;College of Medicine,Yangzhou University,Yangzhou 225009,China;Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases,Yangzhou University,Yangzhou 225009,China;College of Food Science and Engineering,Qingdao Agricultural University,Qingdao 266109,China)

机构地区：[1]扬州市食品药品检验检测中心,江苏扬州225001 [2]武警江苏省总队医院,江苏扬州225003 [3]扬州大学医学院,江苏扬州225009 [4]扬州大学江苏省中西医结合老年病防治重点实验室,江苏扬州225009 [5]青岛农业大学食品科学与工程学院,山东青岛266109

出　　处：《扬州大学学报（农业与生命科学版）》2024年第4期42-48,共7页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：山东省自然科学基金面上项目(ZR2020MB101);扬州市重点研发计划(社会发展)项目(YZ2023068);扬州大学大学生科创基金资助项目(X20220741)。

摘　　要：为研究无花果枝条内生真菌Alternaria sp.QDFB-2菌丝体的化学成分,采用硅胶柱层析、Sephadex LH-20凝胶柱层析、反相柱层析和高效液相色谱等方法对Alternaria sp.QDFB-2菌丝体粗浸膏进行分离纯化,利用核磁共振谱(NMR)和质谱(MS)等现代波谱技术、结合文献比对方法确定化合物的结构;采用MTT法进行化合物的体外细胞毒活性评价。结果表明:从Alternaria sp.QDFB-2菌丝体粗浸膏中分离纯化得到7种化合物:alternariol(1)、tentoxin(2)、dihydrotentoxin(3)、cyclo(Gly-Pro)(4)、cyclo(4-hydroxy-Pro-Pro)(5)、cyclo(S-Leu-S-N-methyl-Phe)(6)和uridine(7)。体外细胞毒活性评价显示,化合物1和7对SMMC-7721细胞具有中等增殖抑制活性,其IC50值分别为50.47μg·mL^(-1)(195.62μmol·L^(-1))和42.50μg·mL^(-1)(174.18μmol·L^(-1));化合物1对A549细胞也具有中等的增殖抑制活性,浓度为100μg·mL^(-1)时抑制率为52.12%;阳性对照顺铂的IC50值分别为3.46μg·mL^(-1)(11.53μmol·L^(-1))和5.12μg·mL^(-1)(17.06μmol·L^(-1))。Alternaria sp.QDFB-2菌丝体的化学成分丰富,值得深入研究。In order to investigate the chemical constituents in the mycelium of Alternaria sp.QDFB-2,an endophytic fungus isolated from the branch of Ficuscarica L.,isolation and purification of the crude extract from the mycelium of Alternaria sp.QDFB-2 was carried out by silica gel column chromatography,Sephadex LH-20 gel column chromatography.reversed phase column chromatography and high-performance liquid chromatography.The structures of obtained compounds were then determined by modern spectroscopic techniques such as nuclear magnetic resonance spectroscopy(NMR)and mass spectrometry(MS)as well as by comparison with literatures.The in vitro cytotoxicity of the compounds was evaluated by MTT method.Seven compounds were obtained from the crude extract of the mycelium from Alternaria sp.QDFB-2,including alternariol(1),tentoxin(2),dihydrotentoxin(3),cyclo(Gly-Pro)(4),cyclo(4-hydroxy-Pro-Pro)(5),cyclo(S-Leu-S-N-methyl-Phe)(6),and uridine(7).The in vitro cytotoxicity evaluation showed that compounds 1 and 7 could inhibit the proliferation of SMMC-7721 cells with IC_(50)values of 50.47μg·mL^(-1)(195.62μmol·L^(-1))and 42.50μg·mL^(-1)(174.18μmol·L^(-1)),respectively.Compound 1 exhibited moderate proliferation inhibitory activity against A549 cells with an inhibition rate of 52.12%at the concentration of 100μg·mL^(-1),while the IC_(50)values of the positive control cisplatin were 3.46μg·mL^(-1)(11.53μmol·L^(-1))and 5.12μg·mL^(-1)(17.06μmol·L^(-1)),respectively.The mycelium of Alternaria sp.QDFB-2 contains rich chemical substances,which is worthy of further research.

关 键 词：无花果 内生菌 Alternaria sp. 化学成分 细胞毒活性 

分 类 号：S663.3[农业科学—果树学] R284.2[农业科学—园艺学]