硫氢化钠对脑缺血再灌注大鼠脑皮质损伤的影响及机制  

Effect and mechanism of sodium hydrosulfide on cerebral cortical injury in rats with cerebral ischemia-reperfusion

作　　者：李颖虹[1] 左月 杨坤丽 任衍开 李东亮[1,2] LI Yinghong;ZUO Yue;YANG Kunli;REN Yankai;LI Dongliang(School of Basic Medical Sciences,Xinxiang Medical University,Xinxiang 453003,Henan Province,China;Sanquan Medical College,Xinxiang Medical University,Xinxiang 453000,Henan Province,China;Institute of Basic Medicine and Forensic Medicine,North Sichuan Medical College,Nanchong 637000,Sichuan Province,China)

机构地区：[1]新乡医学院基础医学院,河南新乡453003 [2]新乡医学院三全学院,河南新乡453000 [3]川北医学院基础医学与法医学研究所,四川南充637000

出　　处：《新乡医学院学报》2024年第10期904-910,共7页Journal of Xinxiang Medical University

基　　金：河南省教育厅高等学校重点科研项目(编号:19A310022);南充市校科技战略合作专项(编号:22SQT0040);新乡医学院三全学院校级立项课题(编号:XJKT201909)。

摘　　要：目的探讨硫氢化钠(NaHS)对脑缺血再灌注大鼠脑皮质损伤的影响及机制。方法将80只雄性SD大鼠按照随机数字表法分为假手术组、NaHS组、缺血再灌注组和缺血再灌注+NaHS组,每组20只。缺血再灌注组和缺血再灌注+NaHS组大鼠制备缺血再灌注模型,缺血再灌注+NaHS组大鼠在脑缺血后腹腔给予NaHS 50μmol·kg^(-1),缺血再灌注组大鼠在脑缺血后腹腔给予等体积生理盐水;假手术组和NaHS组只分离出血管不插线栓,NaHS组大鼠分离出血管后腹腔给予NaHS 50μmol·kg^(-1),假手术组大鼠分离出血管后腹腔给予等体积生理盐水。造模24 h后,采用改良神经系统严重程度评分(mNSS)评估4组大鼠神经功能缺损情况,氯化三苯基四氮唑染色法观察4组大鼠脑组织梗死情况,酶联免疫吸附法检测4组大鼠缺血侧脑皮质组织中促炎因子白细胞介素-1β(IL-1β)和白细胞介素-18(IL-18)水平,Western blot法检测4组大鼠缺血侧脑皮质中核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)表达,免疫荧光法检测4组大鼠缺血侧脑皮质小胶质细胞活化情况。结果假手术组和NaHS组大鼠脑组织呈均匀橘红色,缺血再灌注组和缺血再灌注+NaHS组大鼠缺血侧大脑皮层和纹状体有苍白的梗死区。缺血再灌注组和缺血再灌注+NaHS组大鼠脑梗死面积显著大于假手术组和NaHS组,缺血再灌注+NaHS组大鼠脑梗死面积显著小于缺血再灌注组(P<0.05)。缺血再灌注组和缺血再灌注+NaHS组大鼠mNSS评分显著高于假手术组和NaHS组,缺血再灌注+NaHS组大鼠mNSS评分显著低于缺血再灌注组(P<0.05)。缺血再灌注组和缺血再灌注+NaHS组大鼠缺血侧脑皮质中NLRP3蛋白相对表达量显著高于假手术组和NaHS组,缺血再灌注+NaHS组大鼠缺血侧脑皮质中NLRP3蛋白相对表达量显著低于缺血再灌注组(P<0.05)。缺血再灌注组和缺血再灌注+NaHS组大鼠缺血侧脑皮质中IL-1β和IL-18水平显著高于假手�Objective To explore the effect and mechanism of sodium hydrosulfide(NaHS)on cerebral cortical injury in rats with cerebral ischemia-reperfusion.Methods A total of 80 male SD rats were divided into a sham operation group,an NaHS group,an ischemia-reperfusion group,and an ischemia-reperfusion+NaHS group using a random number table method,with 20 rats in each group.The rats in the ischemia-reperfusion group and ischemia-reperfusion+NaHS group were used to prepare ischemia-reperfusion models.The rats in the ischemia-reperfusion+NaHS group were given 50μmol·kg^(-1)NaHS intraperitoneally after cerebral ischemia,while the rats in the ischemia-reperfusion group were given an equal volume of physiological saline intraperitoneally after cerebral ischemia.The rats in the sham operation group and NaHS group only had vessels isolated but not occluded with threads.The rats in the NaHS group were given 50μmol·kg^(-1)NaHS intraperitoneally after vascular detachment,while the rats in the sham operation group were given an equal volume of physiological saline intraperitoneally after vascular detachment.Twenty-four hours after modeling,the neurological deficits of rats in the four groups were evaluated by using the modified neurological severity score(mNSS),the cerebral infarction of rats in the four groups was observed aftertriphenyltetrazolium chloride staining,the levels of pro-inflammatory cytokines interleukin-1β(IL-1β)and interleukin-18(IL-18)in theischemiccerebralcortical tissues of rats in the four groups were detected by using enzyme-linked immunosorbent assay,the expression of nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3)in the ischemic cerebral cortical tissues of rats in the four groups was detected by using Western blot,and the activity of microglia in the ischemic cerebral cortical tissues of rats in the four groups was detected by using immunofluorescence.Results The brain tissues of rats in the sham operation group and the NaHS group showed uniform orange red color,while the rats in

关 键 词：硫氢化钠 脑缺血再灌注 小胶质细胞活化 脑皮质损伤 炎症 核苷酸结合寡聚化结构域样受体蛋白3 

分 类 号：R329.21[医药卫生—人体解剖和组织胚胎学] R363.2[医药卫生—基础医学]