2021年吉林省手足口病的病原构成及柯萨奇病毒A组16型VP1区基因特征分析  

Analysis on pathogen composition of hand-foot-mouth disease and gene characterization of Coxsackie virus group A type 16 VP1 region in Jilin Province in 2021

作　　者：王艺儒 王爽[1] 沈博[1] 赵庆龙[1] 杨显达[1] 侯程程 WANG Yi-ru;WANG Shuang;SHEN Bo;ZHAO Qing-long;YANG Xian-da;HOU Cheng-cheng(Institute of Virology,Jilin Provincial Center for Disease Control and Prevention(Jilin Institute of Preventive Medicine),Changchun,Jilin 130062,China)

机构地区：[1]吉林省疾病预防控制中心(吉林省预防医学科学院)病毒检验所,吉林长春130062

出　　处：《中国卫生工程学》2024年第4期448-451,共4页Chinese Journal of Public Health Engineering

基　　金：吉林省卫生与健康技术创新项目(2020J096);吉林省卫生健康科技能力科研项目(2021GW014)。

摘　　要：目的了解2021年吉林省手足口病(HFMD)的病原构成及柯萨奇病毒A组16型的VP1区基因特征,为手足口病科学防治提供依据。方法对2021年吉林省手足口病流行的主要病原体构成进行分析,并对优势病原体VP1基因编码区核苷酸序列进行测定和分析。结果278例HFMD患者标本中分离获得78株肠道病毒阳性毒株,阳性率为28.06%,其中CV-A16占35.90%,CV-A6占32.05%,CV-A4占11.54%,CV-A10占6.41%,未分型肠道病毒占14.10%。13株CV-A16阳性毒株中,9株属于B1a基因亚型,4株属于B1b基因亚型。13株CV-A16流行株之间氨基酸和核苷酸同源性分别为97.9%~100.0%和86.7%~100.0%。13株CV-A16流行株的第14、22、23、143、164、251、266及279位点发生了氨基酸突变。结论2021年吉林省HFMD CV-A16,CV-A6两种主要病原体共循环。13株CV-A16流行株B1a和B1b 2个基因亚型共同流行,以B1a基因亚型为主;与2008年青海株相比,共发生了8处氨基酸位点变异。Objective To understand the pathogenic composition of hand-foot-mouth disease(HFMD)and gene charac-teristics of VP1 region of Coxsackie virus group A type 16 in Jilin Province in 2021,so as to provide reference for scientific prevention and treatment of HFMD.Methods The main epidemic pathogenic composition of HFMD in Jilin Province in 2021 was analyzed,and the nucleotide sequence of the VP1 coding region of the dominant pathogen was determined and an-alyzed.Results 78 enterovirus positive strains were isolated from 278 HFMD patients,with a positive rate of 28.06%.Among them,CV-A16 accounted for 35.90%,CV-A6 accounted for 32.05%,CV-A10 accounted for 6.41%,CV-A4 accounted for 11.54%and untyped enterovirus accounted for 14.10%.Among the 13 CV-A16 positive strains,9 belonged to the Bla gene subtype and 4 belonged to the Blb gene subtype.The amino acid and nucleotide homology among the 13 endemic CV-A16 strains were 97.9%-100.0%and 86.7%-100.0%,respectively.Amino acid mutations were observed at sites 14,22,23,143,164,251,266 and 279 of 13 CV-A16 strains.Conclusion Two pathogens of HFMD,CV-A16 and CV-A6,were co-circulating in Jilin Province in 2021.Two gene subtypes,Bla and Blb,were co-prevalent among 13 CV-A16 strains,mainly Bla gene subtype.There were 8 amino acid site mutations in Qinghai strain compared with 2008.

关 键 词：手足口病 柯萨奇病毒A组16型 VP1编码区 

分 类 号：R373.23[医药卫生—病原生物学] R225.1[医药卫生—基础医学]