电感耦合等离子体-质谱法检测血浆中的铟  

Determination of indium in plasma by inductively coupled plasma-mass spectrometry

作　　者：李燕[1] 卿郅聪 何宗剑 宁攀良 LI Yan;QING Zhi-cong;HE Zong-jian;NING Pan-liang(Institute for Physical&Chemical Testing,Guangxi Zhuang Autonomous Region Institute for the Prevention and Treatment of Occupational Disease,Nanning,Guangxi 530021,China)

机构地区：[1]广西壮族自治区职业病防治研究院理化检测所,广西南宁530021

出　　处：《中国卫生工程学》2024年第4期461-464,共4页Chinese Journal of Public Health Engineering

基　　金：广西壮族自治区卫生健康委员会科技研究计划课题(Z20170551)。

摘　　要：目的通过研究不同稀释液条件的检测灵敏度,建立血浆中铟测定的电感耦合等离子体质谱法(ICP-MS)。方法将1 ml血浆用0.2%HNO_(3)-0.05%Triton X-100(体积分数)稀释液定容至10 ml,利用ICP-MS法测定血浆中的铟浓度。结果血浆样品在测定范围内具有良好的线性关系,相关系数为0.9994,检出限为0.0001μg/L,最低检出浓度为0.001μg/L。血浆中铟平均加标回收率为98.3%~108.0%,批内精密度为0.5%~4.5%,批间精密度为4.5%。对15名正常对照人群和15名某铟矿工人血浆样中的铟含量进行检测,结果显示正常人群血浆样中铟浓度平均值为0.026μg/L,铟接触工人血浆样中的铟浓度平均值为0.390μg/L。结论采用ICP-MS法直接稀释检测血浆中的铟,该方法检出限低,灵敏度高,精密度好,可用于血浆中铟浓度的测定。Objective To establish an inductively coupled plasma mass spectrometry(ICP-MS)for the determination of indium in plasma by studying the sensitivity of different diluents.Methods 1 ml plasma was filled to 10 ml with 0.2%HNO_(3)-0.05%Triton X-100(V/V)diluent,and the concentration of indium in plasma was determined by ICP-MS.Results The plasma samples had a good linear relationship in their reasonable range,with the correlation coefficient of 0.9994,with the detection limit of 0.0001μg/L,and the minimum detectable concentration of 0.001μg/L.The average spiked recoveries of indium in plasma were 98.3%-108.0%,the intra-batch precision was 0.5%-4.5%,and the inter-batch precision was 4.5%.The average concentration of indium in plasma samples of normal control population(n=15)was 0.026μg/L,and that of indium miners(n=15)was 0.390μg/L.Conclusion The ICP-MS can be used for the determina-tion of indium concentration in plasma through detecting indium in directly diluted plasma with low detection limit,high sensitivi-ty and good precision.

关 键 词：铟 血浆 电感耦合等离子体质谱法 

分 类 号：R446.1[医药卫生—诊断学] O657.63[医药卫生—临床医学]