四种猪毛菜族植物GLDP及其启动子的克隆与分析  

Cloning and Analysis of GLDP and Its Promoters from Four Salsoleae Species

作　　者：古再丽努尔·库尔班 彭鹏 席昱欣 闻志彬[1,2,6] Guzailinuer KUERBAN;PENG Peng;XI Yuxin;WEN Zhibin(State Key Laboratory of Desert and Oasis Ecology,Key Laboratory of Ecological Security and Sustainable Development in Arid Areas,Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences,Urumqi,830011;Xinjiang Key Laboratory of Stress to Tolerance Plant Resources Conservation and Resistance Gene Utilization,Urumqi,830011;University of Chinese Academy of Sciences,Beijing,100049;College of Life Sciences,Xinjiang Agricultural University,Urumqi,830052;Sino-Tajikistan Joint Laboratory for Conservation and Utilization of Biological Resources,Urumqi,830011;The Specimen Museum of Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences,Urumqi,830011)

机构地区：[1]荒漠与绿洲生态国家重点实验室,干旱区生态安全与可持续发展重点实验室,中国科学院新疆生态与地理研究所,乌鲁木齐830011 [2]新疆抗逆植物基因资源保育与利用重点实验室,乌鲁木齐830011 [3]中国科学院大学,北京100049 [4]新疆农业大学生命科学学院,乌鲁木齐830052 [5]中国-塔吉克斯坦生物资源保育与利用联合实验室,乌鲁木齐830011 [6]中国科学院新疆生态与地理研究所标本馆,乌鲁木齐830011

出　　处：《基因组学与应用生物学》2024年第8期1364-1378,共15页Genomics and Applied Biology

基　　金：国家自然科学基金项目(31970354);新疆维吾尔自治区“天山英才”培养计划(2023TSYCCX0090)共同资助。

摘　　要：甘氨酸脱羧酶(glycine decarboxylase,GDC)是光呼吸代谢途径的关键酶,P蛋白亚基是其脱羧单元。该亚基由小基因家族GLDP编码。GLDP家族成员在C_(3)植物的所有光合细胞中表达,而在C_(3)-C_(4)中间型植物和C_(4)植物中仅在维管束鞘细胞中表达。为了深入了解猪毛菜族(Salsoleae)不同光合类型物种GLDP表达调控的分子机制,本研究基于全基因组数据对该族C_(3)植物天山猪毛菜(Salsola junatovii)、C_(3)-C_(4)中间型植物松叶猪毛菜(Oreosalsola laricifolia)和白枝猪毛菜(Oreosalsola arbusculiformis)以及C_(4)植物木猪毛菜(Xylosalsola arbuscula)的GLDP及其启动子进行克隆与分析,结果表明这四种植物GLDP家族均只有一个成员,它们具有相似的基因结构、保守基序和结构域。GLDP在这些植物根、茎和叶中均有表达,其中在叶中表达量最高,其次是茎和根。基于GLDP在猪毛菜族和其他4个已公布的代表属植物叶中的转录表达丰度分析表明,与C_(3)植物相比,GLDP转录表达丰度在C_(3)-C_(4)中间型植物和C_(4)植物中逐渐降低。此外,利用松叶猪毛菜子叶瞬时转化体系分别对与GUS表达载体融合的上述4种植物的GLDP启动子进行活性分析发现,天山猪毛菜GLDP启动子在子叶所有光合组织中均表达;与7 d子叶相比,松叶猪毛菜、白枝猪毛菜和木猪毛菜GLDP启动子仅在14 d子叶维管束鞘细胞中特异表达。本研究结果为深入研究猪毛菜族不同光合类型物种GLDP组织特异性表达机理提供了参考依据。Glycine decarboxylase(GDC)is a key enzyme in photorespiration metabolic pathway,and P protein subunit is its decarboxylase unit.This subunit is encoded by the small gene family GLDP.GLDP family members are expressed in all photosynthetic cells in C_(3)plants,but only in bundle sheath cells in C_(3)-C_(4)intermediate and C_(4)plants.In order to understand the molecular mechanism of GLDP expression regulation in different photosynthetic types of Salsoleae species,in this study,based on genome-wide data,GLDP and their promoters were cloned and analyzed from the C_(3)plant Salsola junatovii,C_(3)-C_(4)intermediate plants Oreosalsola laricifolia and O.arbusculiformis,C_(4)plant Xylosalsola arbuscula.The results showed that all four species of GLDP family have only one member,and they have similar gene structure,conserved motifs and domains.GLDP was expressed in roots,stems and leaves of these plants,with the highest expression in leaves,followed by stems and roots.Based on the analysis of GLDP transcriptional expression abundance in the leaves of Salsoleae and other four published representative genera,the transcriptional expression abundance of GLDP gradually decreased in C_(3)-C_(4)intermediate plants and C_(4)plants compared with C_(3)plants.In addition,the GLDP promoter activity of these four plants fused with GUS expression vector was analyzed by using the transient transformation system in the cotyledon of Oreosalsola laricifolia,and it was found that the GLDP promoter of Salsola junatovii was expressed in all photosynthetic tissues of the cotyledon.Compared with of 7 d cotyledon,GLDP promoter of Oreosalsola laricifolia,O.arbusculiformis and Xylosalsola arbuscula was specifically expressed only in 14 d cotyledon bundle sheath cells.The search results provided a reference for further study on the tissue specific expression mechanism of GLDP in different photosynthetic types of Salsoleae.

关 键 词：甘氨酸脱羧酶 GLDP 猪毛菜族 C_(3)-C_(4)中间型植物 C_(4)途径 

分 类 号：Q943.2[生物学—植物学]