粟负泥虫成虫触角转录组及OBPs基因家族分析  

Analysis of Antennal Transcriptome and OBPs Gene Family in Adults of Oulema tristis

作　　者：刘佳[1] 曹艺轩 白辉[1] 王永芳[1] 马继芳[1] 刘磊[1] 董志平[1] 李志勇[1] LIU Jia;CAO Yixuan;BAI Hui;WANG Yongfang;MA Jifang;LIU Lei;DONG Zhiping;LI Zhiyong(Institute of Millet Crops,Hebei Academy of Agriculture and Forestry Sciences,Key Laboratory of Ministry of Agriculture and Rural Affairs for Genetic Improvement and Utilization for Featured Coarse Cereals(Co-construction by Ministry and Province),Key Research Laboratory of Minor Cereal Crops of Hebei Province,Shijiazhuang,050035;Graduate School,Hebei North University,Zhangjiakou,075132)

机构地区：[1]河北省农林科学院谷子研究所,农业农村部特色杂粮遗传改良与利用重点实验室(部省共建),河北省杂粮研究重点实验室,石家庄050035 [2]河北北方学院研究生学院,张家口075132

出　　处：《基因组学与应用生物学》2024年第8期1400-1412,共13页Genomics and Applied Biology

基　　金：河北省农林科学院基本科研业务费包干制项目(HBNKY-BGZ-02-RCPY-10);河北省农林科学院科技创新人才队伍建设项目(C21R0401)共同资助;国家现代农业产业技术体系项目(CARS-06-14.5-A25)。

摘　　要：粟负泥虫(Oulema tristis)是谷子上的重要害虫,对谷子的产量和品质均构成了严重威胁。气味结合蛋白(odorant binding proteins,OBPs)作为昆虫嗅觉识别系统中与气味分子结合的重要蛋白,在成虫定位寄主植物及交配行为中发挥着重要作用。本研究通过转录组测序,构建了粟负泥虫成虫触角转录组数据库,挖掘粟负泥虫成虫触角中潜在的OBPs基因家族,并对其编码蛋白的理化性质、保守基序、系统发育及表达量进行了分析。结果表明,本研究得到了一个准确性高、拼接完整度好且功能注释较好的高质量的触角转录组数据库。共鉴定出32个OtriOBPs基因,其编码92~184个氨基酸,蛋白分子的质量为10.07~21.19 kDa。不同分支上OtriOBPs的Motif组成有所差异,但位于同一个分支上的OtriOBPs具有相同的Motif组成。32个OtriOBPs在整个进化树上分布,且相对集中,与光肩星天牛(Anoplophora glabripennis)、灭字脊虎天牛(Xylotrechus quadripes)和苹果小吉丁(Agrilus mali)存在多个直系同源基因对。筛选到了12个在成虫触角中高表达的基因,其中,OtriOBP9基因的相对表达量最高,在雄虫触角中的表达量显著高于雌虫的,可能与雄虫寻找配偶有关,实时定量PCR(real time quantitative PCR,RT-qPCR)的结果与转录组数据一致。本研究为进一步探究粟负泥虫OBPs的功能奠定基础,同时也为后续开发基于嗅觉行为调控的绿色防控技术提供重要的理论依据。Oulema tristis,an important pest of foxtail millet,poses a serious threat to the yield and quality of foxtail millet.Odorant binding proteins(OBPs)is an important protein that binds to odorant molecules in the olfactory sensory system of insects,and plays an important role in adult positioning host plants and mating behavior.In this study,a transcriptome database of the antennae of Oulema tristis adults was constructed by transcriptome sequencing,and the potential OBPs gene family in the antennae of Oulema tristis adults were excavated,and their physicochemical properties,conserved Motifs,phylogenies and expression levels were analyzed.The results showed that a high-quality antennal transcriptome database with high accuracy,good splicing integrity and good functional annotation was obtained.32 OtriOBPs genes were identified,encoding 92~184 amino acids with a protein molecular mass of 10.07~21.19 kDa.The Motif composition of OtriOBPs on different branches was different,but OtriOBPs on the same branch has the same Motif composition.The 32 OtriOBPs were distributed in the whole phylogenetic tree and the distribution was relatively concentrated.There were multiple orthologous gene pairs with Anoplophora glabripennis,Xylotrechus quadripes and Agrilus mali.12 genes were highly expressed in the antennae of Oulema tristis adults by gene expression analysis.Among them,OtriOBP9 gene showed the highest relative expression level,with a significantly higher expression level in male antennae compared to female antennae.It may be related to the male of Oulema tristis looking for a mate.The results of real time quantitative PCR(RT-qPCR)were consistent with the transcriptome data.This study lays a foundation for further exploring the functions of OBPs from Oulema tristis,and also provides an important theoretical basis for the subsequent development of green prevention and control technology based on olfactory behavior regulation.

关 键 词：粟负泥虫 触角 转录组测序 气味结合蛋白(OBPs) 生物信息学分析 

分 类 号：S433.5[农业科学—农业昆虫与害虫防治]