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作 者:Gong Xihui Zhang Manlin Wang Wenna Sun Zhichao Liu Chunshuang
出 处:《China Petroleum Processing & Petrochemical Technology》2024年第3期24-32,共9页中国炼油与石油化工(英文版)
基 金:the Shandong Provincial Natural Science Foundation(No.ZR2019MEE038,ZR202110260011);the Fundamental Research Funds for the Central Universities(No.19CX02038A)。
摘 要:A total of 14 halophilic hydrocarbon-degrading strains were isolated from crude oil-contaminated sites,using petroleum as the sole carbon and energy source.Among these,four highly efficient strains were selected to create the mixed bacterial agent XH-1.These four strains were identified through 16S rRNA gene-based sequencing as belonging to Acinetobacter,Bacillus paramycoides,Rhodococcus sp.,and Enterobacter sp.,respectively.The optimal cultivation time for the mixed consortium XH-1 was found to be 48 h,and a nitrogen-phosphorus molar ratio of 10:1 was determined to be beneficial for crude oil degradation.XH-1 showed notable crude oil degradation even at a salinity of up to 30 g/L,with little inhibition observed at sulfide concentrations as high as 150 mg/L and initial oil concentrations of 500 mg/L.Gas chromatography analysis revealed that XH-1 was able to efficiently degrade C9–C29 n-alkanes.Moreover,a bio-contact oxidation reactor enhanced by XH-1 showed promising results in treating oilfield wastewater.These findings suggest that XH-1 can be applied for the treatment of oilfield wastewater.
关 键 词:crude oil degradation mixed strains oilfield wastewater degradation characteristics
分 类 号:X172[环境科学与工程—环境科学] X741
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