多种检测方法在安徽口岸入境人员疟疾监测中的联合应用  

Combined Application of Multiple Testing Methods in Malaria Surveillance of Individuals Entering Anhui Province

作　　者：俞保圣 许佳 石泉 周勇 叶向光 YU Bao-Sheng;XU Jia;SHI Quan;ZHOU Yong;YE Xiang-Guang(Anhui International Travel Health Care Center(Hefei Customs Port Clinic),Hefei 230000;Hefei Customs,Hefei 230000)

机构地区：[1]安徽国际旅行卫生保健中心(合肥海关口岸门诊部),合肥230000 [2]合肥海关,合肥230000

出　　处：《中国口岸科学技术》2024年第9期31-37,共7页China Port Science and Technology

基　　金：海关总署科研项目(2022HK090)。

摘　　要：为了解安徽口岸入境人员疟疾流行情况,本研究对2023年来自非洲和东南亚等疟疾流行国家和地区的重点入境人员进行主动监测,运用快速诊断试剂检测(Rapid Diagnostic Reagent Test,RDT)、镜检及荧光定量PCR等方法进行联合检测分析。结果表明:在780例入境人员中共检测出3例疟疾样本,其中1例显示RDT阳性,镜检为恶性疟;2例显示RDT阳性,镜检均为阴性,但荧光定量PCR检测均为阳性,结合临床症状和流行病学史分析,3例均感染疟疾。多种疟疾检测方法的联合应用,可以避免单一检测方法产生的假阴性结果,及时发现输入性病例,有效防止我国疟疾输入再传播,充分发挥海关守护国门安全的职责和使命。To assess the prevalence of malaria among individuals entering Anhui Province,this study conducted proactive surveillance in 2023 on key entrants from malaria-endemic regions such as Africa and Southeast Asia.A combination of rapid diagnostic reagent testing(RDT),microscopic examination and fluorescence quantitative PCR was employed for analysis.The results revealed that a total of 3 malaria samples were detected out of 780 entrants,among which 1 case was RDT-positive with microscopic examination confirming falciparum malaria;and 2 cases were RDT-positive with negative microscopic results,but positive fluorescence quantitative PCR results.Combined with clinical symptoms and epidemiological investigation,all three cases were confirmed as malaria infection.The joint application of multiple malaria detection methods helps avoid false-negative results generated by a single test method,ensures timely detection of imported cases,effectively preventing the re-transmission of imported malaria in China,and reinforces the vital role of the Customs in safeguarding national health security.

关 键 词：疟疾 入境人员 镜检 快速诊断试剂检测 荧光定量PCR 

分 类 号：R531.3[医药卫生—内科学] R181.3[医药卫生—临床医学]