基于磷脂酰肌醇3-激酶/蛋白激酶B/雷帕霉素靶蛋白通路探讨大株红景天注射液对缺氧H9C2心肌细胞损伤的影响  

Effects of sofren injection on hypoxic H9C2 cardiomyocytes based on PI3K/AKT/mTOR pathway

作　　者：薛莲 李平 于雪 张淑静 杨阳 XUE Lian;LI Ping;YU Xue;ZHANG Shujing;YANG Yang(Graduate School of Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学研究生院,100029 [2]北京中医药大学中医学院,100029 [3]北京中医药大学第三附属医院心内科

出　　处：《环球中医药》2024年第9期1742-1748,共7页Global Traditional Chinese Medicine

基　　金：国家自然科学基金(81960852)。

摘　　要：目的探讨大株红景天注射液对缺氧H9C2心肌细胞的影响及保护机制。方法取H9C2心肌细胞缺氧造模,实验分为正常组、模型组、大株红景天注射液组(简称:SI组)、尼可地尔组。采用RT-qPCR检测磷脂酰肌醇3-激酶(phosphoinositide 3-kinase,PI3K)、蛋白激酶B(protein kinase B,AKT)、雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)的mRNA;免疫蛋白印记法检测PI3K、AKT、mTOR、p-PI3K、p-AKT、p-mTOR的蛋白表达;流式细胞仪检测细胞凋亡水平。结果与模型组比较,SI组及尼可地尔组PI3K、AKT、mTOR的mRNA表达均显著降低(P<0.05)。与模型组比较,SI组和尼可地尔组PI3K、AKT、mTOR蛋白表达无明显差异(P>0.05)。经磷酸化处理后,与模型组比较,SI组和尼可地尔组p-PI3K、p-AKT、p-mTOR的蛋白表达显著下降(P<0.05);与尼可地尔组相比,SI组p-PI3K、p-AKT的蛋白表达下降(P<0.05),p-mTOR的蛋白含量无明显差异(P>0.05)。结论大株红景天注射液通过下调PI3K/AKT/mTOR通路,起到了抑制缺氧心肌细胞过度自噬,改善心肌细胞损伤的作用。Objective To investigate the effect and protective mechanism of sofren injection in hypoxic H9C2 cardiomyocytes.Methods H9C2 cardiomyocytes were simulated by hypoxia.The experiment was divided into normal group,model group,sofren injection group(SI group)and nicodil group.mRNA levels of phosphoinositide 3-kinase(PI3K),protein kinase B(AKT)and mammalian target of rapamycin(mTOR)were detected by RT-qPCR.The protein expression of PI3K,AKT,mTOR,p-PI3K,p-AKT and p-mTOR were detected by Western Blot.The level of apoptosis was detected by flow cytometry.Results Compared with the model group,the mRNA expressions of PI3K,AKT and mTOR in the SI group and the nicodil group were significantly decreased(P<0.05).Compared with the model group,there were no significant differences in PI3K,AKT and mTOR protein expressions between the SI group and the nicodil group(P>0.05).After phosphorylation,the protein expressions of p-PI3K,p-AKT and p-mTOR in SI the group and the nicodil group were significantly decreased compared with the model group(P<0.05).Compared with the nicodil group,the protein expressions of p-PI3K and p-AKT in the SI group were decreased(P<0.05),while the protein content of p-mTOR was not significantly different(P>0.05).Conclusion Sofren injection can suppress excessive autophagy of hypoxic cardiomyocytes and ameliorate cardiac cell damage via downregulating the PI3K/AKT/mTOR pathway.

关 键 词：大株红景天注射液 心绞痛 缺氧心肌细胞 磷脂酰肌醇3-激酶/蛋白激酶B/雷帕霉素靶蛋白通路 自噬 

分 类 号：R285.5[医药卫生—中药学]