白黎芦醇对多囊卵巢综合征大鼠瘦素表达及胰岛素抵抗、自噬相关因子调节的研究  

Study on the expression of Leptin and the regulation of insulin resistance and autophagy related factors in PCOS rats

作　　者：贾三三 薛东芳 谭小青 苏璇 JIA Sansan;XUE Dongfang;TAN Xiaoqing;SU Xuan(Reproductive Medicine Department,Linfen People’s Hospital,Linfen,Shanxi 041000,China)

机构地区：[1]临汾市人民医院生殖医学科,山西临汾041000

出　　处：《中国优生与遗传杂志》2024年第8期1600-1604,共5页Chinese Journal of Birth Health & Heredity

基　　金：临汾市人民医院院级科研基金项目(T20210512143)。

摘　　要：目的探究白黎芦醇对多囊卵巢综合征(PCOS)大鼠血清瘦素(leptin)表达、胰岛素抵抗、自噬相关因子的调节作用。方法研究对象选取健康大鼠39只,随机分为对照组、PCOS组、治疗组,对照组正常喂养,PCOS组、治疗组建立PCOS模型,治疗组使用白藜芦醇。观察各组大鼠卵巢、胰腺β细胞病理学变化及leptin表达、胰岛素抵抗、自噬相关因子表达水平。结果对照组有不同发育阶段的卵泡,颗粒细胞形态完整,呈现8~9层,可见卵母细胞;PCOS组囊性扩张卵泡增多,颗粒细胞层变薄,可见闭锁卵泡以及卵母细胞消失;治疗组颗粒细胞层变厚,囊性扩张卵泡减少,成熟卵泡增多;PCOS组β细胞明显减少,治疗组β细胞数量较PCOS组增加,略低于对照组;各组空腹胰岛素、胰岛素抵抗、leptin、氧化应激指标、p62、LC3I、LC3Ⅱ蛋白相对表达比较,差异有统计学意义,PCOS组空腹胰岛素、HOMA-IR、leptin、MDA、p62、LC3Ⅱ水平高于对照组及治疗组,治疗组高于对照组,PCOS组HOMA-β、HOMA-s%、GSH、SOD、LC3I水平低于对照组及治疗组,治疗组低于对照组(P<0.05)。结论白藜芦醇能下调PCOS大鼠leptin表达,改善其胰岛素抵抗,抑制自噬,从而改善其卵巢及胰岛β细胞病理学变化。Objective To explore the regulatory effects of veriratol on serum leptin expression,endoplasmic reticulum stress,insulin resistance and autophagy related factors in PCOS rats.Methods 39 healthy rats were randomly divided into control group,PCOS group and treatment group.The control group was fed normally,the PCOS group and treatment group were treated with PCOS model,and the treatment group was treated with resveratrol.The pathological changes of beta cells in ovary and pancreas and the expression levels of leptin,endoplasmic reticulum stress.Insulin resistance and autophagy related factors were observed.Results In the control group,there were follicles at different stages of development,and the morphology of granulosa cells was complete,showing 8-9 layers,and oocytes were visible.In the PCOS group,cystic dilated follicles were increased,the granulosa cell layer was thin,and atretic follicles and oocytes were visible.In the treatment group,the granulosa cell layer was thickened,cystic dilated follicles were reduced,and mature follicles were increased.The number ofβcells in the COS group was significantly decreased,and the number ofβcells in the treatment group was higher than that in the PCOS group,which was slightly lower than that in the control group.There were statistically significant differences in fasting insulin,insulin resistance,leptin,oxidative stress indices,p62,LC3I and LC3Ⅱprotein relative expressions among all groups.Fasting insulin,HOMA-IR,leptin,MDA,p62 and LC3Ⅱlevels in PCOS group were higher than those in control group and treatment group,and those in treatment group were higher than those in control group.The levels of HOMA-β,HOMA-s%,GSH,SOD and LC3I in PCOS group were lower than those in control group and treatment group,and the treatment group was lower than control group(P<0.05).Conclusion Resveratrol can down-regulate the expression of leptin in PCOS rats,improve the insulin resistance,inhibit the autophagy,and improve the pathological changes of ovarian and pancreaticβcells.

关 键 词：白黎芦醇 PCOS 瘦素 胰岛素抵抗 自噬 

分 类 号：R711.75[医药卫生—妇产科学]