基于便携式近红外光谱仪的测定新生儿足底血精氨酸实验研究  

Experimental study on the determination of arginine in newborn foot blood based on portable near infrared spectrometer

作　　者：孙岱 周玉侠 沈峰[1] 孙茂 张娟 SUN Dai;ZHOU Yuxia;SHEN Feng;SUN Mao;ZHANG Juan(Laboratory Department,Shandong Provincial Maternal and Child Health Care Hospital Affiliated to Qingdao University,Jinan,Shandong 250014,China;Shandong JinzhangLongxiang Intelligent Technology Co.,Ltd.,Jinan,Shandong 250014,China;Health Management Center,Shandong First Medical University Affiliated Provincial Hospital,Jinan,Shandong 250021,China)

机构地区：[1]青岛大学附属山东省妇幼保健院检验科,山东济南250014 [2]山东金璋隆祥智能科技有限责任公司,山东济南250014 [3]山东第一医科大学附属省立医院健康管理中心,山东济南250021

出　　处：《中国优生与遗传杂志》2024年第8期1697-1701,共5页Chinese Journal of Birth Health & Heredity

基　　金：山东省医药卫生科技发展计划项目(202211000939)。

摘　　要：目的应用便携式近红外光谱仪对新生儿足底血精氨酸含量进行定量研究。方法利用近红外漫反射光谱技术收集115例足底血精氨酸的光谱数据,利用偏最小二乘法(PLS)、多元散射校正(MSC)+二阶导数(SD)等预处理技术,构建了足底血精氨酸的定量分析模型。结果通过配对t检验和误差分析,发现精氨酸的近红外预测值与参照值之间没有明显的差别,且具有较好的预报效果;其定量模型校正集相关系数R=0.9835,均方根误差RMSEC=4.5761,内部交叉验证决定系数R2=0.9670。结论定量分析模型能够快速的检测出足底血精氨酸的含量,结果准确可靠;近红外技术可以用于精氨酸定量分析模型的建立,为足底血精氨酸测定提供新的分析方法。Objective To study the content of arginine in neonatal blood plantar by portable near-infrared spectrometer.Methods The data of 115 cases of arginine in plantar blood were collected by near-infrared diffuse reflectance spectroscopy.The model of quantitative analysis of arginine in plantar blood was established by partial least square method(PLS)and multiplicative scatter correction(MSC)+second derivative(SD)pretreatment.Results Through pairwise t test and error analysis,it was found that there was no obvious difference between the predicted value and the reference value of arginine,and the predicted value was good.The correlation coefficient of its quantitative model correction set R=0.9835,root mean square error RMSEC=4.5761,and internal cross validation determination coefficient R2=0.9670.Conclusion Quantitative analysis model can quickly detect the content of arginine in blood of plantar,and the results are accurate and reliable.NIR can be used to establish a quantitative analysis model of arginine.

关 键 词：近红外光谱技术 精氨酸 偏最小二乘法 交叉验证均方差 定量分析模型 

分 类 号：R722.1[医药卫生—儿科] R446.11[医药卫生—临床医学]