2023-2024年山东省南四湖地区禽流感感染情况监测结果分析  

Analysis of surveillance results of avian influenza infection in Nansi lake area,Shandong province from 2023 to 2024

作　　者：李立博 黄友光 梁玉民[1] 姜文国[1] 刘伟[1] 焦伯延[1] 王青川 LI Li-bo;HUANG You-guang;LIANG Yu-min;JIANG Weng-guo;LIU Wei;JIAO Bo-yan;WANG qing-chuan(Infectious Disease Prevention Section,Jining City Center for Disease Control and Prevention,Shandong 272000,China;Department of Laboratory Medicine,Jining Dermatology Control Hospital;Department of Respiratory Medicine,Jining Municipal Hospital)

机构地区：[1]济宁市疾病预防控制中心传染病防制科,山东272000 [2]济宁市皮肤病防治院检验科 [3]济宁市市直机关医院呼吸内科

出　　处：《预防医学论坛》2024年第7期508-512,共5页Preventive Medicine Tribune

基　　金：山东省公共卫生体系建设研究课题(GWY20231)。

摘　　要：目的分析山东省南四湖地区候鸟、家禽(养殖场、屠宰场)及周边人群禽流感病毒感染情况。方法于南四湖流域的济宁市任城区、鱼台县、微山县自然保护区内设置11个候鸟标本采样点,2023年1月-2024年3月在候鸟迁徙季节,共采集596份新鲜候鸟粪便样本;在济宁市选择4个调查点(邹城、金乡、兖州、汶上),2010年1月-2024年3月,共采集家禽标本3726份;2023年1月-2024年3月,累计纳入湖区暴露人群687名、活禽市场禽职业暴露人群104名及对照人群73名,采集其血清标本。采用荧光定量PCR检测候鸟、家禽标本中流感病毒,血凝抑制试验检测血清标本中不同亚型流感病毒抗体。结果候鸟标本中流感病毒携带率为2.3%(14/596);活禽市场家禽标本中流感病毒的携带率为9.0%(337/3726),进一步分型显示H5、H7、H9亚型流感病毒阳性检出率分别为0.4%(14/3726)、0.4%(14/3726)和4.7%(174/3726);血清学调查显示候鸟栖息地居住人群和禽职业暴露人群H5N1、H9N2亚型流感病毒的血清抗体阳性率高于对照人群,其中禽职业暴露人群较对照人群对H9N2禽流感病毒有更高的感染风险(OR=1.68,95%CI:1.239~2.994,P<0.05)。结论山东省南四湖地区活禽市场中禽流感病毒阳性率较高,其中H9亚型的流感病毒占比最高;湖区人群和禽类职业暴露人群感染H5和H9亚型流感病毒的风险较高。Objective To analyze avian influenza virus infections in migratory birds,poultry(farms and slaughterhouses)and surrounding populations in the Nansi lake area of Shandong province.Methods A total of 11migratory bird specimen sampling sites were set up in Rencheng district,Yutai county and Weishan county nature reserves in Jining city in the Nansi lake basin,and 596fresh migratory bird fecal samples were collected from January 2023to March 2024during the migratory season;Four survey sites(Zoucheng,Jinxiang,Yanzhou and Wenshang)were selected in Jining city,and a total of 3726poultry specimens were collected from January 2010to March 2024.From January 2023to March 2024,a total of 687exposed individuals from the lake area,104occupationally exposed workers from live poultry markets,and 73control individuals were included,and their serum specimens were collected.Fluorescence quantitative PCR was used to detect influenza viruses in migratory birds and poultry specimens,and hemagglutination inhibition test was used to detect antibodies to different subtypes of influenza viruses in serum specimens.Results The prevalence of avian influenza virus in specimens from migratory birds was 2.3%(14/596).The prevalence of avian influenza virus in environmental specimens from live poultry markets was 9.0%(337/3726),in which the prevalence against H5,H7,and H9subtypes was 0.4%(14/3726),0.4%(14/3726),and 4.7%(174/3726),respectively.Serological investigation showed that the residents living around the Nansi lake and the poultry-exposed workers had higher seroprevalence against H5N1and H9N2than that of the general control population.In particular,individuals with occupational exposure to poultry had a higher risk of infection with H9N2avian influenza virus than the control group(OR=1.68,95%CI:1.239-2.994,P<0.05).Conclusion The rate of positive detection for avian influenza virus in live poultry markets in the South Four Lakes area of Shandong Province is relatively high,with the H9subtype accounting for the highest proportion;the risk of

关 键 词：禽流感 南四湖 候鸟 家禽 人群 高致病性禽流感病毒 

分 类 号：R373.13[医药卫生—病原生物学] R535[医药卫生—基础医学]