Effects of acupuncture and moxibustion on chromatin remodeling-related enzymes in the colon tissue of rats with Crohn disease  

针灸对克罗恩病大鼠结肠组织染色质重塑相关酶的影响

作　　者：LI Yanting GUO Yajing ZHAO Jimeng SUN Yi GUO Sen SHI Yin 李燕婷;郭娅静;赵继梦;孙怡;郭森;施茵(Seventh People’s Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200137,China;Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shanghai Research Institute of Acupuncture and Meridian,Shanghai 200030,China)

机构地区：[1]Seventh People’s Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200137,China [2]Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China [3]Shanghai Research Institute of Acupuncture and Meridian,Shanghai 200030,China

出　　处：《Journal of Acupuncture and Tuina Science》2024年第5期353-362,共10页针灸推拿医学（英文版）

基　　金：国家自然科学基金项目,No.82174503,No.81674069.

摘　　要：Objective:To investigate the regulatory effects of acupuncture and moxibustion on chromatin remodeling complex core catalytic subunit of Brahma-related gene 1(Brg1),histone deacetylase(HDAC)3,HDAC9,and males absent on the first(MOF)in the colon tissue of rats with Crohn disease(CD).Methods:Using the random number table method,60 male Sprague-Dawley rats were divided into 5 groups,including a normal group,a model group,an acupuncture group,a medicinal cake-insulated moxibustion group,and an acupuncture-moxibustion group,with 12 rats in each group.CD rat models were prepared using 2,4,6-trinitrobenzene sulfonic acid(TNBS)in all groups except the normal group.The normal and model groups received no interventions.In the acupuncture group,rats were intervened with acupuncture at bilateral Zusanli(ST36)and Shangjuxu(ST37),20 min/session,once a day.The medicinal cake-insulated moxibustion group received medicinal cake-insulated moxibustion at Qihai(CV6)and bilateral Tianshu(ST25)with 2 cones per point per session,once a day.The acupuncture-moxibustion group received both acupuncture and moxibustion interventions simultaneously.Each intervention was performed for 10 consecutive days.Observations included general condition,disease activity,macroscopic damage,and pathological changes in the rat’s colon tissue.Realtime fluorescence quantitative polymerase chain reaction was used to measure the mRNA expression of chromatin remodeling-related enzymes Brg1,HDAC3,and HDAC9,while Western blotting detected the protein expression of Brg1,HDAC3,HDAC9,and MOF in rat’s colon tissue.Results:The model group showed significantly increased diarrhea score,occult blood score,macroscopic damage score of colon tissue,and colon macroscopic damage index(CMDI)score,as well as elevated mRNA expression levels of HDAC3 and HDAC9,protein expression levels of HDAC3,HDAC9,and MOF,and decreased mRNA and protein expression levels of Brg1 compared to the normal group(P<0.01).In contrast,compared to the model group,the diarrhea score,occult blood sco目的:探讨针灸对克罗恩病(CD)大鼠结肠组织染色质重构复合物核心催化亚基(Brg1)、组蛋白去乙酰化酶3(HDAC3)、组蛋白去乙酰化酶9(HDAC9)、组蛋白乙酰基转移酶(MOF)的调节作用。方法:将60只雄性Sprague-Dawley大鼠根据随机数字表法分成5组,包括正常组、模型组、针刺组、隔药灸组和针灸组,每组12只。除正常组外,其余4组采用2,4,6-三硝基苯磺酸(TNBS)制备CD大鼠模型。正常组和模型组不干预;针刺组针刺双侧足三里和上巨虚,每日1次,每次20 min;隔药灸组接受隔药饼灸气海和双侧天枢,每日1次,每次每穴2壮;针灸组同时接受与隔药灸组和针刺组相同的两种干预方法。各组均连续干预10 d。观察各组大鼠大体情况、疾病活动度、结肠组织宏观损伤及病理学改变。采用实时荧光定量聚合酶链反应检测各组大鼠结肠组织染色质重塑相关酶Brg1、HDAC3及HDAC9的mRNA表达;免疫印迹法检测各组大鼠结肠组织Brg1、HDAC3、HDAC9及MOF的蛋白表达情况。结果:与正常组比较,模型组大鼠腹泻评分、隐血评分、结肠组织宏观损伤评分、结肠黏膜损伤指数(CMDI)评分、结肠组织HDAC3、HDAC9的mRNA以及HDAC3、HDAC9、MOF的蛋白表达显著增高(P<0.01),Brg1的mRNA和蛋白表达均显著降低(P<0.01)。与模型组相比,针刺组、隔药灸组和针灸组大鼠腹泻评分、隐血评分、结肠组织宏观损伤评分、CMDI评分以及结肠组织HDAC3、HDAC9的mRNA和HDAC3、HDAC9、MOF的蛋白表达均降低(P<0.05或P<0.01),Brg1的mRNA和蛋白表达均显著增高(P<0.01)。结论:隔药灸和针刺无论单独使用还是合用均可调节CD大鼠结肠组织染色质重塑相关酶Brg1、HDAC3、HDAC9和MOF的异常表达,减轻CD大鼠的结肠炎症。

关 键 词：Acupuncture Therapy Moxibustion Therapy Medicinal Cake-insulated Moxibustion Crohn Disease Inflammatory Bowel Diseases Chromatin Remodeling RATS 

分 类 号：R2-03[医药卫生—中医学]