高原地区不同程度冠状动脉狭窄与EGR3、VEGF蛋白的相关性分析  

Analysis of correlation between EGR3 or VEGF protein and different degrees of coronary stenosis in plateau area

作　　者：史梦真 白玉婷[2] 周白丽[2] 苏晓灵[2] 刘彦民[2] 郭文路 魏晓娟[2] 乌更苏雅 SHI Mengzhen;BAI Yuting;ZHOU Baili;SU Xiaoling;LIU Yanmin;GUO Wenlu;WEI Xiaojuan;WUGENG Suya(Graduate School of Qinghai University,Xining Qinghai 810016;Department of Cardiology,Qinghai Provincial People's Hospital,Xining Qinghai 810007,China;Clinical Laboratory,Qinghai Provincial People's Hospital,Xining Qinghai 810007,China)

机构地区：[1]青海大学研究生院,青海西宁810016 [2]青海省人民医院心内科,青海西宁810007 [3]青海省人民医院检验科,青海西宁810007

出　　处：《实用心电学杂志》2024年第5期438-443,453,共7页Journal of Practical Electrocardiology

基　　金：青海省卫生健康系统指导性计划课题(2021-wjzdx-07)。

摘　　要：目的对不同程度冠状动脉(简称冠脉)狭窄与人早期生长反应因子3(early growth response 3,EGR3)、血管内皮生长因子(vascular endothelial growth factor,VEGF)等蛋白因子的相关性进行探讨,同时筛选出与EGR3、VEGF相关性最强的蛋白因子,并探讨高原地区快速诊断冠心病以及评估冠脉狭窄程度潜在的新生物标志物。方法选取青海省人民医院(海拔2260 m)心内科住院患者172例,根据冠脉狭窄程度将其分为病例组126例(包括动脉粥样硬化组38例、中度狭窄组71例、重度狭窄组17例)和对照组46例。检测EGR3、VEGF等蛋白因子并进行统计学分析,再借助京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)分析,探讨高原地区快速诊断冠心病以及评估冠脉狭窄程度潜在的新生物标志物。结果动脉粥样硬化组、中度狭窄组、重度狭窄组的人单核细胞/巨核细胞主要组织相容性复合体受体1(human monocyte/megakaryocyte major histocompatibility complex receptor 1,MMR1)、EGR3、人血栓素B2(thromboxane B2,TXB2)、人血清反应因子(serum response factor,SRF)、人血小板活化因子(platelet activating factor,PAF)、VEGF、人cAMP反应元件结合蛋白(cyclic-AMP response element binding protein,CREB)、人血小板生成素(thrombopoietin,TPO)、人血小板反应蛋白/凝血酶敏感蛋白1(thrombospondin 1,THBS1)水平与对照组比较,差异均有统计学意义(均P<0.05)。Spearman相关性分析显示,血清VEGF与TXB2、SRF、PAF、EGR3、CREB、TPO、THBS1均呈正相关(r=0.460、0.644、0.539、0.462、0.740、0.702、0.673,均P<0.05),以血清CREB与VEGF相关性最强。血清EGR3与MMR1、TXB2、SRF、PAF、VEGF、CREB、TPO、THBS1均呈正相关(r=0.405、0.682、0.780、0.700、0.462、0.686、0.551、0.245,均P<0.05),以血清SRF与EGR3相关性最强。通过KEGG分析得出,在通路hsa04010中,SRF在VEGF的下游,调控增殖与分化;在通路hsa04926中,CREB在VEGF的上游,调控血管生�Objective To investigate the correlation between different degrees of coronary stenosis and protein factors such as human early growth response 3(EGR3)and vascular endothelial growth factor(VEGF),and to screen out the protein factors with the strongest correlation with EGR3 and VEGF.To explore the potential new biomarkers for rapid diagnosis of coronary heart disease(CHD)and assessment of coronary stenosis degree in plateau area.Methods A total of 172 patients hospitalized in the Department of Cardiology,Qinghai Provincial Peoples Hospital(2260 m above sea level)were selected,and divided into case group(n=126,including 38 cases in the atherosclerosis group,71 cases in the moderate stenosis group and 17 cases in the severe stenosis group)and control group(n=46)according to the degree of coronary stenosis.Protein factors such as EGR3 and VEGF were detected,and statistically analyzed.By using Kyoto Encyclopedia of Genes and Genomes(KECG)analysis,the potential new biomarkers for rapid diagnosis of CHD and evaluation of coronary stenosis degree in plateau area were explored.Results The indexes of human monocyte/megakaryocyte major histocompatibility complex receptor 1(MMR1),EGR3,human thromboxane B2(TXB2),human serum response factor(SRF),human platelet activating factor(PAF),VEGF,human cyclic-AMP response element binding protein(CREB),human thrombopoietin(TPO),thrombospondin 1(THBS1)all significantly varied from the atherosclerosis group,moderate stenosis group or severe stenosis group to the control group(all P<0.05).Spearman correlation analysis showed that serum VEGF was positively correlated with TXB2,SRF,PAF,EGR3,CREB,TPO and THBS1(r=0.460,0.644,0.539,0.462,0.740,0.702,0.673,all P<0.05).Among them,the correlation between serum CREB and VEGF was the strongest.Serum EGR3 was positively correlated with MMR1,TXB2,SRF,PAF,VEGF,CREB,TPO and THBS1(r=0.405,0.682,0.780,0.700,0.462,0.686,0.551,0.245,all P<0.05).Among them,the correlation between serum SRF and EGR3 was the strongest.KEGG analysis showed that SRF regulated p

关 键 词：冠心病 冠状动脉狭窄 人早期生长反应因子3 血管内皮生长因子 

分 类 号：R541.4[医药卫生—心血管疾病]