Multi-gene-based investigation on the molecular phylogeny of the hypotrichous family Strongylidiidae(Protista,Ciliophora),with notes on the ontogeny of a new genus and new species  

作　　者：Wenya Song Shijing Zhang Yuqing Li Honggang Ma Qiyu Li Xiaotian Luo Khaled A.S.Al-Rasheid Hunter N.Hines Xiaoteng Lu 

机构地区：[1]Institute of Evolution&Marine Biodiversity,Ocean University of China,Qingdao 266003,China [2]Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China [3]Weishan Special Aquaculture Base,Jining 277600,China [4]Zoology Department,King Saud University,11451 Riyadh,Saudi Arabia [5]Harbor Branch Oceanographic Institute,Florida Atlantic University,Fort Pierce,Florida 34946,USA [6]Department of Biology,Shenzhen MSU-BIT University,Shenzhen 518172,China

出　　处：《Marine Life Science & Technology》2024年第3期442-461,共20页海洋生命科学与技术（英文）

基　　金：funded by the Science&Technol-ogy Innovation Project of Laoshan Laboratory(No.LSKJ202203201);National Natural Science Foundation of China(No.32030015,32370475);Researchers Supporting Project(No.RSP2024R10)King Saud University,Riyadh,Saudi Arabia;Guangdong Province Youth Innovative Talent Project 2022(2022KQNCX107).

摘　　要：Ciliates in the subclass Hypotrichia have long been difficult to classify as they are one of the most polymorphic and highly differentiated groups,leading to their systematics remaining unresolved.Phylogenetic relationships within the hypotrich family Strongylidiidae have been ambiguous due to discordance between the morphological and genetic data.In this study,a new strongylidiid genus Heterouroleptus is established,mainly based on the novel mode of origin of the ventral cirral rows:left ventral cirral row(LVR)originates from frontal-ventral-transverse cirral anlagen(FVTA)Ⅲ(anterior portion),IV(middle portion),and V(rear portion);right ventral cirral row comes from the entire FVTA VI.A new species,Hetero-uroleptus weishanensis gen.nov.,sp.nov.,is investigated along with the morphometric and molecular data from a population of Strongylidium wuhanense.Eight new sequences and nuclear gene markers(single-gene and multi-gene)are provided to analyze the phylogenetic relationships of strongylidiids,with the COI gene utilized to uncover further genetic information at species level and below.The results reveal that:(1)Strongylidiidae is monophyletic and has a close relationship with Dorsomarginalia;(2)Heterouroleptus gen.nov.forms a clade that is sister to all the other strongylidiids;(3)Hemiamphisiella Foissner,1988 and Pseudouroleptus Hemberger,1985 should not be synonyms,and both genera should be subdivided due to their variable morphological characteristics;(4)LVR originating from three anlagen is a plesiomorphy of Strongylidiidae.The discovery of the origin of the LVR not only contributes to the establishment of the genus Heterouroleptus,but also helps to improve the diagnosis of the family Strongylidiidae.

关 键 词：CILIATES Cytochrome c oxidase subunit I gene MORPHOGENESIS Phylogeny·Ribosomal RNA gene Taxonomy 

分 类 号：Q95[生物学—动物学]