Molecular mechanism of a coastal cyanobacterium Synechococcus sp.PCC 7002 adapting to changing phosphate concentrations  

作　　者：Qiao-Wei Sun Yu Gao Jordan Wang Fei-xue Fu Cheng-Wen Yong Shuang-Qing Li Hai-Long Huang Wei-Zhong Chen Xin-Wei Wang Hai-Bo Jiang 

机构地区：[1]School of Marine Sciences,Ningbo University,Ningbo 315211,China [2]Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai),Zhuhai 519080,China [3]School of Life Sciences,Central China Normal University,Wuhan 430079,China [4]Department of Biological Sciences,University of Southern California,Los Angeles,CA 90089,USA

出　　处：《Marine Life Science & Technology》2024年第3期562-575,共14页海洋生命科学与技术（英文）

基　　金：the support from Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai,No.SML2021SP204);funded by the National Natural Science Founda-tion of China(Grant Nos.32170108,and 42188102);the Science and Technology Innovation 2025 Major Project of Ningbo City(Grant No.2022Z189);the Ningbo Public Welfare Science and Technology Program(Grant Nos.2023S068,2023S040).

摘　　要：Phosphorus concentration on the surface of seawater varies greatly with different environments,especially in coastal.The molecular mechanism by which cyanobacteria adapt to fluctuating phosphorus bioavailability is still unclear.In this study,transcriptomes and gene knockouts were used to investigate the adaptive molecular mechanism of a model coastal cyanobacterium Synechococcus sp.PCC 7002 during periods of phosphorus starvation and phosphorus recovery(adding sufficient phosphorus after phosphorus starvation).The findings indicated that phosphorus deficiency affected the photosynthesis,ribosome synthesis,and bacterial motility pathways,which recommenced after phosphorus was resupplied.Even more,most of the metabolic pathways of cyanobacteria were enhanced after phosphorus recovery compared to the control which was kept in continuous phosphorus replete conditions.Based on transcriptome,54 genes potentially related to phosphorusdeficiency adaptation were selected and knocked out individually or in combination.It was found that five mutants showed weak growth phenotype under phosphorus deficiency,indicating the importance of the genes(A0076,A0549-50,A1094,A1320,A1895)in the adaptation of phosphorus deficiency.Three mutants were found to grow better than the wild type under phosphorus deficiency,suggesting that the products of these genes(A0079,A0340,A2284–86)might influence the adaptation to phosphorus deficiency.Bioinformatics analysis revealed that cyanobacteria exposed to highly fluctuating phosphorus concentrations have more sophisticated phosphorus acquisition strategies.These results elucidated that Synechococcus sp.PCC 7002 have variable phosphorus response mechanisms to adapt to fluctuating phosphorus concentration,providing a novel perspective of how cyanobacteria may respond to the complex and dynamic environments.

关 键 词：Synechococcus sp PCC 7002 Phosphorus fluctuation CYANOBACTERIA ・Gene knockout Molecular mechanism 

分 类 号：Q178.53[生物学—水生生物学] P714.5[生物学—普通生物学]