转录组分析低氧胁迫对鲫鳃组织基因表达影响  

Transcriptome analysis of the effects of hypoxic stress ongene expressi on in gill tissues of crucian carp

作　　者：侯智广 陈力 邢梦超 常瑞泽 吴成宾 HOU Zhiguang;CHEN Li;XING Mengchao;CHANG Ruize;WU Chengbin(Ocean College of Hebei Agricalture University,Qinhuangdao 066000,China;Hebei Academy of Marine and Fishery Sciences,Qinhuangdao 066003,China)

机构地区：[1]河北农业大学海洋学院,河北秦皇岛066000 [2]河北省海洋与水产科学研究院,河北秦皇岛066003

出　　处：《河北渔业》2024年第10期20-23,29,F0002,共6页

基　　金：国家自然科学基金项目(32303030);河北省自然科学基金(C2021204089,V1654588161890,236Z6701G);河北农业大学人才引进科研基金(YJ2020032);河北省现代农业产业技术体系淡水养殖创新团队名优特种类繁育及绿色高效养殖(HBCT2023230205)。

摘　　要：为探究鲫(Carassius auratus)鳃组织对低氧的适应性变化,使用RNA-seq技术对低氧(溶解氧0.6 mg/L±0.2 mg/L,水温16℃±0.5℃)处理0 h和96 h的鲫的鳃组织进行转录组测序,共获得40.22 Gbp Clean Base,每个样本93%~96%的碱基质量值大于Q30。差异分析共获得7142个差异基因(|log 2FoldChange|≥1且FDR<0.05)。GO功能富集分析表明,差异表达基因主要在DNA复制启动、MCM复合体等功能显著富集。KEGG通路富集分析表明,差异基因主要在DNA复制、VEGF信号通路、坏死型凋亡等通路中被显著富集。综上所述,vegf、vegfb、hba、hbb、mcm2—mcm6、ripk3等基因是鲫96 h低氧适应的关键基因。In order to investigate the adaptive changes of crucian carp(Carassius auratus)to hypoxia,gill tissues treated with hypoxia(dissolved oxygen 0.6 mg/L±0.2 mg/L,water temperature 16℃±0.5℃)for 0 h and 96 h and were subjected to transcriptome sequencing by RNA-seq,and a total of 40.22 Gbp Clean Base was obtained,and 93%~96%of bases in each sample had a mass value greater than Q30.Differential analysis yielded a total of 7142 differential genes(|log 2FoldChange|≥1 and FDR<0.05).GO functional enrichment analysis showed that the differentially expressed genes were significantly enriched mainly in DNA replication initiation,MCM complex,etc.KEGG pathway enrichment analysis showed that the differentially expressed genes were mainly in DNA replication,VEGF signaling pathway,necrotic apoptosis and other functions.In summary,vegf,vegfb,hba,hbb,mcm2—mcm6,ripk3 and other genes are the key genes for 96 h hypoxia adaptation in crucian carp.

关 键 词：鲫(Carassius auratus) 低氧胁迫 转录组 

分 类 号：S917.4[农业科学—水产科学]