宁都黄鸡禽白血病和鸡白痢的监测与分析  

Monitoring and Analysis of Avian Leukemia and White Dysentery in Ningdu Yellow Chickens

作　　者：田玉玲 雷小文 谢华亮 曾锐 肖莉群 钟敏 黄美峰 TIAN Yuling;LEI Xiaowen;XIE Hualiang;ZENG Rui;XIAO Liqun;ZHONG Min;HUANG Meifeng(Ganzhou Institute of Animal Husbandry and Fisheries,341401,Ganzhou,Jiangxi,PRC;Ganzhou City Animal Husbandry Development and Animal Disease Prevention and Control Center,341099,Ganzhou,Jiangxi,PRC)

机构地区：[1]赣州市畜牧水产研究所,江西赣州341401 [2]赣州市畜牧业发展和动物疫病防控中心,江西赣州341099

出　　处：《江西科学》2024年第5期949-952,1131,共5页Jiangxi Science

基　　金：赣州市科技计划项目(赣市科发﹝2020﹞46号)。

摘　　要：为了解宁都黄鸡禽白血病和鸡白痢的流行情况,于2023年在宁都黄鸡规模化养殖场N1、宁都黄鸡散养场N2共采集样本866份,其中387份血清样品和479份蛋清样品。采用酶联免疫吸附试验(ELISA)对禽白血病p27抗原和J亚型禽白血病病毒(ALV-J)抗体进行检测,采用平板凝集试验(SPA)对鸡白痢抗体进行检测。结果表明,蛋清中禽白血病p27抗原总阳性率为7.1%,宁都黄鸡规模化养殖场N1种蛋带毒率较低,曾祖代、祖代和父母代种蛋带毒率均≦5.0%。血清中ALV-J抗体总阳性率为54.4%,鸡白痢抗体总阳性率为7.0%,宁都黄鸡规模化养殖场N1的鸡群此阶段鸡白痢抗体被检测为全阴性。宁都黄鸡存在感染禽白血病和鸡白痢现状,规模化养殖通过多种净化措施对降低禽白血病和鸡白痢的阳性率具有良好效果,各散养场需及时建立有效净化方案,以减少禽白血病和鸡白痢带来的经济损失。In order to understand the prevalence of avian leukemia and white dysentery in Ningdu Yellow Chickens,a total of 866 samples,including 387 serum samples and 479 egg white samples,were collected from Ningdu Yellow Chickens large-scale breeding farm N1 and Ningdu Yellow Chickens free-range farm N2 in 2023.The p27 antigen and ALV-J subtype of avian leukemia virus(ALV-J)were detected by enzyme-linked immunosorbent assay(ELISA),and the white dysentery antibody was detected by plate agglutination assay(SPA).The results showed that the total positive rate of avian leukemia p27 antigen in egg white was 7.1%,with the infection rate of hatching eggs being lower in Ningdu Yellow Chicken large-scale farm N1,where the great-grandparents,grandparents and parent egg infection rate were all≦5.0%.The overall positive rate of ALV-J antibody in serum was 54.4%,and the overall positive rate of chicken white dysentery antibody was 7.0%,with the white dysentery antibody of chickens from N1 of Ningdu Yellow Chicken large-scale breeding farm being completely negative at this stage.The presence of avian leukemia and white dysentery in Ningdu Yellow Chickens was detected.The large-scale breeding showed a good effect on reducing the positive rate of avian leukemia and white dysentery through various purification measures;Therefore,it’s imperative for free-range farms to establish an effective purification scheme promptly to reduce the economic loss caused by avian leukemia and white dysentery.

关 键 词：宁都黄鸡 禽白血病 鸡白痢 监测 

分 类 号：S831.7[农业科学—畜牧学]